高性能イオン交換クロマトグラフィー用ポリマーの開発とそのヒト血清アルブミン成分分析への応用
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概要
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The versatility of 4-vinylpyridinium (4VP-EG-Q) and quaternized dimethylaminomethylstyrene (PDAMS-Q) polymers synthesized by suspension copolymerization as column packings for high performance ion-exchange chromatography was described. The proposed polymer columns afforded resolution of human/bovine serum albumin components, such as mercaptalbumin and nonmercaptalbumin. It was suggested that the physicochemical structure on the external surface of the ion-exchangers was very important in the resolution. The characteristics of 4VP-EG-Q column were effectively applied to the investigation of the metal-albumin complexes, whereas a rapid analysis of mercaptalbumin in the human serum using the PDAMS-Q column was performed by isocratic elution.
- 公益社団法人日本薬学会の論文
- 1993-05-25
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