High Performance Liquid Chromatography of ^<99m>Tc Labeled Human Serum Albumin Using an N-Methylpyridinium Polymer Column

概要

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Human serum albumin (HSA) labeled with ^<99m>Tc (^<99m>Tc-HSA) was analyzed by high performance liquid chromatography using a 4-vinylpyridinium polymer column which specifically resolved albumin components such as human mercaptalbumin (HMA), human non-mercaptalbumins (HNA), etc. The ^<99m>Tc-HSA radiochromatogram revealed that ^<99m>Tc-HSA consisted of several components. The radiochromatographic profile was similar to that of ^<99m>Tc-HMA prepared with ^<99m>Tc and separated HMA. This suggested that HMA participated mainyl in ^<99m>Tc-labeling of HSA. When HSA was labeled with a stoichiometric concentration of ^<99m>Tc, the HMA peak was significantly decreased and new peaks were revealed by absorbance at 280 nm. From these results, the role of HMA in labeling HSA with ^<99m>Tc was elucidated.
公益社団法人日本薬学会の論文
1992-03-25