高速液体クロマトグラフィーによる化粧品中の代表的な紫外線吸収剤の迅速分析法

概要

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Typical 11 ultraviolet (UV) absorbers in cosmetic products were separated and determined by high performance liquid chromatography. The recommended conditions for the analysis were as follows : column, 4.6 mm i.d.×250 mm, Capcell Pak C_<18> SG (5μm) ; detector, UV spectrophotometer (280 nm) ; column temperature, 40℃ ; mobile phase, H_2O-CH_3OH (15 : 85 v/v) containing 3 mM stearyltrimethyl ammonium chloride ; flow rate, 1.0 ml/min. In the case of the products containing 2-ethylhexyl p-methoxycinnamate (OMC) and /or 4-tert-butyl-4'-methoxydibenzoylmethane (BMD), it was necessary to use H_2O-1,4-dioxane (34 : 66 v/v) containing 6 mM stearyltrimethyl ammonium chloride as the mobile phase in order to separate OMC and BMD. Anthracene and 9,10-dimethylanthracene were used as an internal standard for the H_2O-CH_3OH mobile phase and the H_2O-1,4-dioxane mobile phase, respectively. Calibration curves were linear within a range of 50-150μg/ml for the 11 UV absorbers. Recoveries and reproducibilities of the method were satisfactory. By using this method, typical UV absorbers in several commercial cosmetic products such as lip creams, sun oils, lotions and emulsions were able to be rapidly determined without any interference.
社団法人日本薬学会の論文
1991-09-25