環境水中のミクロシスチンのデイスク型固相による抽出

概要

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The cyanobacteria Microcystis which produces microcystin, as a hepatic toxin, has been found in the eutrophic water area including the source of water supply. The contamination of drinking water with these algae could be presumed. Therefore, the determination of microcystin in water by HPLC using a disk type solid phase extraction was studied. Microcystins-RR, -YR and -LR in the water were treated with Empore disks C-18 and SDB-XD (styrene divinyl benzene polymer). As a result, the SDB-XD disk using 10% H_2O-methanol as an eluent showed good recovery and reproducibility. This method can rapidly treat a water sample about ten times faster than the cartridge type solid phase extraction method. To clean the extract from the water sample, a method with a Sep-Pak silica cartridge which connects two pieces was tried using 0.2% TFA・10% H_2O-methanol as an eluent and this method also showed good recovery. The recovery of microcystin from spiked water was 102.3% for -RR, 103.2% for -YR and 91.2% for -LR. In the analysis of water samples collected from the water area yielding "water bloom", microcystin -RR 0-0.05 μg/l, -YR 0-0.02 μg/l and -LR 0-0.05 μg/l were detected in the water and in the samples collected from the algae cell, microcystin-RR 0.15-3.67 μg/l, -YR 0-1.21 μg/l and -LR 0.07-2.03 μg/l were detected. The release rate of microcystin from the algae to water was about 1%.
社団法人日本薬学会の論文
1997-06-30