TLCによる中毒起因物質としての農薬の迅速スクリーニング法

概要

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When the patients who were suspected to take pesticides were carried into hospital, rapid identification of pesticides was mostly necessary. We investigated the rapid and simple screening method for the identification of pesticides in the case of toxicosis in general hospital using thin layer chromatography (TLC). We selected 46 common pesticides having many toxications. They were analyzed by fluorescent silica gel TLC with four developing solvent systems and three detection methods. As the results, 24 pesticides were detected at less than 0.5 μg by observation under UV radiation. Twelve of 46 pesticides reacted with Dragendorff reagent. Especially paraquat and diquat were highly sensitive to it. 4-(p-nitrobenzyl) pyridine-tetraethylenepentamin reagent reacted to all organophosphorous compounds and their lower detection limits were 0.03-20 μg over a wide range. Standard deviations of Rf values were very small. The good recoveries of pesticides were obtained from spiked serum samples. This method enabled us to identify pesticides as the cause substances of toxicosis rapidly from the serum and returned gastoric lavege solution from patients.
社団法人日本薬学会の論文
1994-02-28