Analysis of Antitumor Properties of Effector Cells Stimulated with a Cell Wall Preparation (WPG) of Bifidobacterium infantis

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Intestinal Bifidobacterium species are thought to be beneficial in animal and human intestines. We studied the mechanisms of Bifidobacteria in antitumor activity using a cell wall preparation (WPG) of B. infantis (Cancer Res., 45,1300,(1985)). WPG enhanced the in vitro antitumor activities of mouse peritoneal exudate cells elicited with proteose-peptone (P-PEC) and thioglycollate broth (TG-PEC), determined by cytostatic ([^3H] thymidine uptake inhibition) and cytolytic ([^3H] uridine release) assays. Tumor necrosis factor-α (TNF-α) and reactive nitrogen intermediates (RNI) play a role in such augmented cytotoxicity, because anti-TNF-α antibody almost completely blocked the increased cytolytic activity of P-PEC in the presence of WPG. Moreover, WPG induced RNI in the supernatant of TG-PEC in a dose-dependent manner. The mRNA expression of several cytokines (IL-1β, IL-6,IL-10,IFN-α and TNF-α) was induced in BALB/c mouse peritoneal cells 3 h after an intraperitoneal injection of WPG (3 h WPG-PEC). However, this expression disappeared from 24 h WPG-PEC, except for that of IFN-α. IFN-γ was not induced. Kinetic studies of the tumor neutralizing activities of the WPG-PECs by means of the in vivo Winn assay revealed that the activity emerged at 1.5 h, became maximal at 3 h and disappeared at 24 h. These results indicated that Bifidobacterial WPG is a Biological Response Modifier (BRM) with characteristics similar to those of other bacterial BRMs.
公益社団法人日本薬学会の論文
1995-01-15

Analysis of Antitumor Properties of Effector Cells Stimulated with a Cell Wall Preparation (WPG) of Bifidobacterium infantis

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