Studies on the Quantitative Autoradiography. I. Radioluminography for Quantitative Autoradiography of ^<14>C

概要

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Evidence is presented to elucidate whether or not quantitative analysis of autoradiographs can be obtained with a ^<14>C-labelled compound by the use of a new type of radiosensor called Imaging plate (IP). Our results showed that a linear relationship between relative intensity of PSL-BG and radioactivity of a given area was obtained. The linearity was maintained in a relatively wide range from 10^1 to 10^5 dpm of radioactivity. About 100 times higher sensitivity of the IP than any commercially available X-ray film was demonstrated for not only ^<14>C-radioactive standard sources but also experimentally obtained ^<14>C-radioactive spots developed on a TLC plate. Results also showed a linear relationship between relative intensity and radioactivity of the specimens in a very wide range from 10^1 to 10^5 dpm/mg after exposure for 7 d. Furthermore, the relationship between PSL-BG and relative exposure (radioactivity×exposure time) was linear when PSL-BG was from 10^1 to 10^5. In addition to the above properties, a combination of IP and BAS2000,a computerized image display system, was able to completely erase the background prior to use.
公益社団法人日本薬学会の論文
1995-01-15