Studies on the Quantitative Autoradiography. II. Radioluminography for Quantitative Autoradiography of ^3H

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The process of obtaining ^3H-autoradiographic (ARG) images has been expected for a long time. A few X-ray films with no protection layer are commercially available, however, they do not give a reliable image which can be quantitated and can give good contrast. We tried to fabricate a ^3H-type sensor which has no protection layer on a highly sensitive sensor, and called it Imaging plate (IP). The IP is composed of one of the specially designed photo-stimulable phosphors containing of BaFX : Eu^<2+> (X=Cl, Br or I) crystals. Results indicated a good contrast and reliable whole-body ARG image of ^3H-labelled glucose with trial IP, which has never been obtained with any X-ray films even if these were subjected to a long exposure time. The ARG image can be displayed by either black-and-white hard copy or a colored one with the digital display representing relative intensity of photostimulated luminescence (PSL) without back ground (BG) or relative intensity concentration ((PSL-BG)/S), where S was equivalent to 100 pixels (1 mm^2). Similarly to the experimental results of ^<14>C, the linear relationship relative intensity and radioactivity of the ^3H standard sources was demonstrated with a very wide range of 10^2 to 10^5 dpm/mg upon the exposure for 7 d. Also the relationship between relative intensity and relative exposure (radioactivity×exposure time) was linear within the latitude of relative intensity 10^1 to 10^5 (PSL-BG). The trial IP was particularly effective for the quantitative autoradiography of TLC plates. After development, a good linearity was demonstrated between PSL intensity and radioactivity for each spot on the TLC plate, in a wide latitude of 1 to 10^4,which is similar to that obtained in the ^<14>C experiments. These data strongly support the practical use of the IP and BAS2000 for processing a computerized image for quantitative analysis of macroautoradiographs obtained with not only ^<14>C-labelled drugs but also ^3H-labelled drugs.
公益社団法人日本薬学会の論文
1995-01-15

Studies on the Quantitative Autoradiography. II. Radioluminography for Quantitative Autoradiography of ^3H

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