Use of Transcriptional-Enhancer Elements Responsive to Ras, Protein Kinase A and Protein Kinase C to Evaluate Inhibitors of Specific Signal Transduction Pathways

概要

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To evaluate ras-mediated signal transduction, we constructed a transient transfection assay system that measures chloramphenicol acetyl transferase activity expressed under the transcriptional-enhancer elements responsive to ras, protein kinase C, and protein kinase A in NIH3T3 cells. Characterization of the assay system with several known activators and inhibitors of signal transduction pathways proved that our system could reliably evaluate agents that affect individual pathways. Pirarubicin ((2"R)-4'-tetrahydropyranyladriamycin, THP), which has recently been found able to reverse ras-transformed cells, appeared to selectively inhibit the ras signal transduction pathway.
公益社団法人日本薬学会の論文
1994-06-15