Apoptosis of NG108-15 Cells Induced by Buprenorphine Hydrochloride Occurs via the Caspase-3 Pathway
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概要
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Apoptosis of NG108-15 neuroblastoma×glioma hydrid cells (NG108-15 cells) is induced by a morphine alkaloid derivative, buprenorphine hydrochloride (Bph). In a previous report, we used various apotosis inhibitors to identify the "death pathway", and found that caspase inhibitors Ac-YVAD-CHO (Ac-Yyr-Val-Ala-Asp-CHO) and Ac-DEVD-CHO (Ac-Asp-Glu-Val-Asp-CHO) did not inhibit this particular apoptosis. Here we tested ZAVD-FMK (Z-Val-Ala-Asp[OMe]-CH_2F) and Z-DEVD-FMK (Z-Asp[OMe]-Glu-[OMe]Val-Asp[OMe]-CH_2F) for their ability to inhibit Bph-induced NG108-15 apoptosis. These tri- or tetra-peptide caspase inhibitors have a fluoromethyl ketone in their C-terminus instead of an aldehyde, and thus are more permeable than Ac-YVAD-CHO and AC-DEVD-CHO. Our observations DNA ladder formatin, cell morphology changes, and caspase-3 activities all indicated that these cell membrane-permeable caspase inhibitors completely inhibited the apoptosis, providing strong evidince that this apoptosis occurs through the caspase cascade "death pathway." Our previous report also showed that pretreatment of NG108-15 cells with TPCK (N-tosyl-L-phenylalanyl chloromethyl ketone) prevented DNA fragmentation and decreased the cell viability in Bph-induced apoptosis. The comparsion of caspase-3 activities in Bph-induced samples with or without TPCK pretreatment revealed that caspase-3 was activated in both samples. Taken together, these results indicate that the Bph-induced apoptosis of NG-108-15 cells occurs via the conventional caspase-dependent death pathway and that TPCK pretreatment results in a DNA ladder-deficient apoptosis.
- 公益社団法人日本薬学会の論文
- 2000-08-01
著者
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Kugawa Fumihiko
Department of Biopharmaceutics, School of Pharmacy, Hyogo University of Health Sciences
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Ueno Akemichi
Department of Hygiene Chemistry, School of Pharmaceutical Sciences, Ohu University
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AOKI Masatada
Department of Biological Pharmaceutical Sciences, College of Pharmacy, Nihon University
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Ueno A
Department Of Biochemistry School Of Dentistry The University Of Tokushima
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Aoki M
Department Of Biological Pharmaceutical Sciences College Of Pharmacy Nihon-university
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Aoki Masatada
Department Of Biological Pharmaceutical Sciences College Of Pharmacy Nihon University
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Kugawa F
Department Of Biological Pharmaceutical Sciences College Of Pharmacy Nihon-university
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Kugawa Fumihiko
Department Of Biological Pharmaceutical Sciences College Of Pharmacy Nihon Univ.
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Ueno Akemichi
Department Of Biochemistry School Of Dentistry The University Of Tokushima
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