Accumulation of Medium Chain Acyl-CoAs during β-Oxidation of Long Chain Fatty Acid by Isolated Peroxisomes from Rat Liver

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We have reported fatty alcohol synthesis accompanied by chain elongation in liver peroxisomes(Biochim.Biophys.Acta, 1346, 38(1997)). In the present experiment, we studied what kind of acyl-CoA(s)destined to be utilized as primer for fatty alcohol synthesis accumulate(s) during peroxisomal β-oxdation. Peroxisomes were prepared from rat liver treated with clofibrate, a peroxisome proliferator, and incubated with[U-^<14>Cl]palmitate, in order to investigate acyl-CoAs after β-oxidation. At 1 mм concentration, MgATP activated β-oxidation, but inhibited β-oxdation at concentrations higher than 1mм. After incubation of peroxisomes with palmitate, various acyl-CoAs were formed. Among medium-chain labelled acyl-CoAs, octanoyl-CoA was mainly detected. These results suggest that octanoyl-CoA accumulates during β-oxidation of palmitate. When peroxisomes were incubated with[9, 10-^3H]palmitate and[9, 10^3H]stearate, among medium-chain acyl-CoAs, octanoyl-CoA and decanoyl-CoA were primarily detected, respectively, suggesting the occurrence of at least 4 cycles of β-oxidation of both fatty acids by peroxisomes.
公益社団法人日本薬学会の論文
2001-06-01