A Sensitive Assay of Human Blood Platelet Cyclic Nucleotide Phosphodiesterase Activity by HPLC Using Fluorescence Derivatization and Its Application to Assessment of Cyclic Nucleotide Phosphodiesterase Inhibitors
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概要
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A selective and sensitive HPLC measurement of 3', 5'-cyclic nucleotide phosphodiesterase (PDE) activity in human platelets using (3, 4-dimethoxyphenyl) glyoxal (DMPG) as a fluorogenic reagent for guanine and its nucleosides and nucleotides is described. cGMP, a substrate for PDE, and GMP, which was produced by the enzyme reaction, are selectively converted by the reaction with DMPG to the fluorescent derivatives. The derivatives were separated by reversed-phase HPLC. Human platelet PDE activity was measured and the inhibitory effects of several compounds were investigated.
- 公益社団法人日本薬学会の論文
- 2001-05-01
著者
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ZAITSU KIYOSHI
Graduate School of Pharmaceutical Sciences Kyushu University
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Ohkura Yosuke
Faculty Of Pharmaceutical Sciences Kyusyu University 62
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OHBA Yoshihito
Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University
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Ohba Yoshihito
Graduate School Of Biomedical Sciences Course Of Pharmaceutical Sciences Nagasaki University
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Ohba Yoshihito
Graduate School Of Pharmaceutical Sciences Kyushu University
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SODA Kyoko
Graduate School of Pharmaceutical Sciences, Kyushu University
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Soda Kyoko
Graduate School Of Pharmaceutical Sciences Kyushu University
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