Chemiluminescence Derivatization of Methylglyoxal Using 2-Aminonicotinic Acid
スポンサーリンク
概要
- 論文の詳細を見る
To develop a sensitive and selective chemiluminometric method for the determination of methylglyoxal, we used 2-aminonicotinic acid as the chemiluminescence derivatization reagent. 2-Aminonicotinic acid reacts with methylglyoxal in an acidic solution at 37℃ for 4 h and gave a chemiluminescence in N, N'-dimethylformamide containing sodium tert-butoxide. The detection limit (blank intensity plus three-times its standard deviation of methylglyoxal) for methylglyoxal is 233 fmol in the reaction mixture.
- 公益社団法人日本薬学会の論文
- 2000-08-01
著者
-
ZAITSU KIYOSHI
Graduate School of Pharmaceutical Sciences Kyushu University
-
OHBA Yoshihito
Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University
-
Ohba Yoshihito
Graduate School Of Biomedical Sciences Course Of Pharmaceutical Sciences Nagasaki University
-
SAKATA Miyuki
Graduate School of Pharmaceutical Sciences, Kyushu University
-
Sakata Miyuki
Graduate School Of Pharmaceutical Sciences Kyushu University
関連論文
- プロエンハンサー基質を用いる製剤中のリパーゼ活性の簡易,迅速な化学発光測定
- Protein Stone Formation in an Intrapancreatic Remnant Cyst After Resection of a Choledochal Cyst
- 抗酸化物質の次亜塩素酸イオン消去能測定のためのシーケンシャルインジェクション-ルミノール化学発光分析法の開発(若手研究者の初論文特集)
- Retrospective Analyses of Atmospheric Polycyclic and Nitropolycyclic Aromatic Hydrocarbons in an Industrial Area of a Western Site of Japan
- Determination of Hydroxylated Polycyclic Aromatic Hydrocarbons in Airborne Particulates by High-Performance Liquid Chromatography with Fluorescence Detection
- Determination of Polyphenols with HPLC-Sensitized Chemiluminescence
- Flow Injection Analysis for Pyrogallol Using 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide as Polyphenol Chemiluminescence Enhancer
- Sequential Injection Analysis with Chemiluminescence Detection for the Antioxidative Activity against Singlet Oxygen
- Determination of free D-aspartic acid, D-serine and D-alanine in the brain of mutant mice lacking D-amino-acid oxidase activity
- Attomole Analysis of Melatonin by Precolumn Derivatization Reversed-Phase Micro-HPLC
- A sensitive internal standard method for the determination of melatonin in mammals using precolumn oxidation reversed-phase high-performance liquid chromatography
- Analysis of Small Amounts of D-Amino Acids and the Study of Their Physiological Functions in Mammals
- Sensitive Determination of D-Amino Acids in Mammals and the Effect of D-Amino-Acid Oxidase Activity on Their Amounts(D-Amino Acid Biosystem)
- Determination of D- and L-enantiomers of threonine and allo-threonine in mammals using two-step high-performance liquid chromatography
- D-Amino acids in mammals and their diagnostic value
- Reversed-Phase HPLC of Monosaccharides in Glycoproteins Derivatized with Aminopyrazine with Fluorescence Detection
- Non-Acidic Matrix for the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of Acid-Labile Compounds
- Selective Masking of Amino Groups during Fluorescence Derivatization in the Presence of a Molecular Imprint Polymer
- Heme-Undecapeptide Labeling on Insulin for the Immunoassay of Insulin with Chemiluminescence Detection
- Chemiluminescence of Polyhydroxyphenol Linked-Calix[4]arenes
- Chemiluminescence Enhancer of Polyphenols, 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide
- A Sensitive Assay of Human Blood Platelet Cyclic Nucleotide Phosphodiesterase Activity by HPLC Using Fluorescence Derivatization and Its Application to Assessment of Cyclic Nucleotide Phosphodiesterase Inhibitors
- Chemiluminescence Derivatization of Methylglyoxal Using 2-Aminonicotinic Acid
- Automated and simultaneous two-dimensional micro-high-performance liquid chromatographic determination of proline and hydroxyproline enantiomers in mammals
- Circadian changes of D-alanine and related compounds in rats and the effect of restricted feeding on their amounts
- Reversible Fluorescence Derivatization of Amino Groups Using Dansylaminomethylmaleic Acid via Its Anhydride
- Lasting Chemiluminescence of 3-Indoleglyoxylyl Chloride and Its Enhancement
- Determination of D-serine and D-alanine in the tissues and physiological fluids of mice with various D-amino-acid oxidase activities using two-dimensional high-performance liquid chromatography with fluorescence detection
- Chemiluminescence Characteristics of N-(4-Substituted Benzyl)isoluminol