Involvement of Reactive Oxygen Species in Hemoglobin Oxidation and Virus Inactivation by 1, 9-Dimethylmethylene Blue Phototreatment
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概要
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The participation of reactive oxygen species(ROS) in virus inactivation by 1, 9-dimethylmethylene blue(DMMB) phototreatment in stroma-free hemoglobin(SFH) was investigated with the use of scavengers, quenchers and enhancer. Virus(R17 bacteriophage) photoinactivation by either activated monomer or dimer DMMB was suppressed by sodium azide(singlet oxygen quencher) and promoted by the substitution of H_2O for deuterium oxide(D_2O), which is known to prolong the lifespan of singlet oxygen. There was no or little effect of mannitol(hydroxyl radical scavenger) and superoxide dismutase(superoxide scavenger) on the photoinactivation. Similar experiments were conducted to investigate the mechanism of methemoglobin(Met-Hb) formation by the activated monomer of DMMB. There was little effect of the singlet oxygen quencher, histidine, or the enhancer, D_2O, on Met-Hb formation. However, rutin, which inhibits not only singlet oxygen but also other ROS, and mannitol supressed the formation of Met-Hb by activated monomer. The addition of superoxide dismutase(SOD) did not inhibit the formation. In contrast to the activity of the DMMB monomer, that of the dimer was inhibited by histidine and enhanced by D_2O. The addition of neither mannitol nor SOD affected Met-Hb formation by activated dimer. These results collectively suggest that virus photoinactivation by the activated monomer and dimer of DMMB as well as Met-Hb formation by the activated dimer proceed via a singlet oxygen mediated pathway. In contrast, singlet oxygen may play a less important role in Met-Hb formation by the activated monomer.
- 社団法人日本薬学会の論文
- 2001-04-01
著者
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Ikebuchi Kenji
Hokkaido Red Cross Center, Japanese Red Cross
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ABE hideki
Hokkaido Red Cross, Blood Center
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IKEBUCHI Kenji
Tokyo Medical University
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IKEDA Hisami
Hokkaido Red Cross, Blood Center
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Abe H
Hokkaido Red Cross Blood Center
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Abe Hideki
早稲田大学理工学総合研究センター
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Abe Hideki
Department Of Chemistry The University Of Tokyo
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Ikeda Hisami
早稲田大学理工学総合研究センター
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Hirayama J
Hokkaido Red Cross Blood Center
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HIRAYAMA Junichi
Hokkaido Red Cross Blood Center
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WAGNER Stephen
Product Development Department, The Jerome H.Holland Laboratory, American Red Cross Blood Services
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Ikebuchi Kenji
Department Of Hematology Nippon Medical School
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Ikeda Hisami
Hokkaido Red Cross Blood Center
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Wagner Stephen
Product Development Department The Jerome H.holland Laboratory American Red Cross Blood Services
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Abe Hideki
Hokkaido Red Cross Blood Center
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Ikebuchi Kenji
Hokkaido Red Cross Blood Center
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Abe H
Department Of Chemistry The University Of Tokyo
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Inokuchi Koiti
Nippon Medical School Tokyo Jpn
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