Involvement of Prostaglandins in Kaolin-Induced Writhing Reaction in Mice

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概要

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• The relationship between the kaolin-induced writhing reaction and production of arachidonate metabolites (PGs) in mice was studied. PGs were released into the peritoneal cavity after intraperitoneal injection (i.p.) of kaolin (2.5 mg/mouse) with a peak at 5 min. About 80% of the total amount was 6-keto-PGF_<1α>. There was a significant correlation (r=0.8237,p<0.001) between the number of writhes and the amount of 6-keto-PGF_<1α>. The writhing reaction induced by kaolin was significantly inhibited by simultaneous injection of soybean trypsin inhibitor (SBTI ; 2.5 mg/mouse) and increased by simultaneous injection of captopril (50μg/mouse). The writhing reaction induced by kaolin which was inhibited by oral administration of indomethacin (1 mg/kg) was restored by exogenous i.p. injection of PGI_2-Na (2-10ng/mouse). Indomethacin, ibuprofen and alminoprofen inhibited the writhing reaction and reduced the level of peritoneal 6-keto-PGF_<1α> in parallel manner. Tiaramide, pentazocine and morphine inhibited the writhing reaction without reducing the revels of 6-keto-PGF_<1α>. These results differentiate the site of action of these analgesics. They suggest that the mechanism of the kaolininduced writhing reaction in mice involves a synergic pain caused by simultaneously released bradykinin and PGI_2. This model is a useful tool which allows differentiation of mode of action of analgesics by simultaneous determination of the writhing response and peritoneal 6-keto-PGF_<1α>.

• 公益社団法人日本薬学会の論文

著者

• Ikeda Katsumi

  Faculty Of Pharmaceutical Sciences Okayama University

• Ohishi Sachiko

  Depertment Of Pharmacology Kitasato University School Of Pharmaceutical Sciences:pharmaceuticals Res

• Ikeda K

  Life Science Laboratories Central Research Laboratories Ajinomoto Co. Inc.:pharmaceuticals Research

• Fujiyoshi T

  Life Science Laboratories Central Research Laboratories Ajinomoto Co. Inc.:pharmaceuticals Research

• FUJIYOSHI Toshio

  Depertment of pharmacology, Kitasato University, School of Pharmaceutical Sciences

• DOZEN Masaharu

  Depertment of pharmacology, Kitasato University, School of Pharmaceutical Sciences

• IKEDA Kenro

  Depertment of pharmacology, Kitasato University, School of Pharmaceutical Sciences

• Dozen Masaharu

  Pharmaceuticals Research Laboratories Fujirebio Inc.

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