PARTICIPATION OF CYTOCHROME b_5 ELECTRON TRANSPORT SYSTEM COUPLED WITH Δ^5-3β-HYDROXYSTEROID DEHYDROGENASE ON CYTOCHROME P-450 MONOOXYGENASE REACTIONS OF GUINEA PIG ADRENAL MICROSOMES

概要

論文の詳細を見る
Not only cytochrome P-450 and cytochrome P-450 reductase, but considerable amount of cytochrome b_5 and cytochrome b_5 reductase were also contained in the adrenal microsomes of guinea pig. Addition of nicotineamide adenine dinucleotide (NADH) stimulates the activities of cytochrome P-450 monooxygenases such as 21-hydroxylase, 17α-hydroxylase and C_<17,20> lyase supported with nicotineamide adenine dinucleotide phosphate (NADPH) as a cofactor. In addition, substrate for Δ^5-3β-hydroxysteroid dehydrogenase plus NAD^+ also stimulate the same cytochrome P-450 monooxygenase activities. These observations were suggesting the participation of cytochrome b_5 electron transport system on cytochrome P-450,monooxygenase (21 -hydroxylase, 17 α-hydroxylase and C_<17,20> lyase) reactions associated with the biosynthesis of corticoids and adrenal androgens. Furthermore, it is also suggesting that cytochrome b_5 electron transport system involves the reaction of Δ^5-3β-hydroxysteroid dehydrogenase.
公益社団法人日本薬学会の論文