Studies on Immunological Assay of Urinary Estrogens. III. A New Latex Agglutination Inhibition Reaction Method

スポンサーリンク

概要

• 論文の詳細を見る

• A new, simple and rapid method for the immunological determination of estrogens in pregnancy urine is described. The principle of this method is based on the latex agglutination inhibition reaction in a solid-solid system. An antibody latex reagent (Ab-Latex) was obtained by sensitizing latex particles with anti-estriol 16-glucuronide antibody which had been raised in a goat. Estriol 16-glucuronide-bound latex reagent (E_3-16-G-Latex) was prepared by the condensation of polyacrylic acid combined with estriol 16-glucuronide (E_3-16-G) and lysine-latex which had been prepared from carboxylate modified latex. Hexamethylenediamine was used for the binding of E_3-16-G to polyacrylic acid. The latex agglutination inhibition tests were performed on opaque glass slides. One drop (30μl) of Ab-Latex suspension followed by one drop of E_3-16-G-Latex suspension was added to one drop of diluted urine sample and mixed thoroughly. The slides were rotated gently for 2 minutes, and a macroscopically visible agglutination inhibition pattern was observed in the presence of an estrogen concentration of 0.1μg/ml (as estriol) or more. The estrogen values in urine can be obtained by multiplying the sensitivity by the highest dilution factor able to give a positive reaction. This test cross-reacted not only with free estriol but also with all free and conjugated estrogens having a free hydroxy group at position 3 in the steroid ring. The urinary estrogen values obtained by this method showed a good correlation with those measured by a radioimmunoassay method (correlation coefficient ; 0.9845,regression line ; y=0.823x-0.614). When this method was compared with a semi-quantitative determination method, the hemagglutination inhibition reaction (E_3 HAIR kit), the results of the two methods were in fairly good agreement.

• 公益社団法人日本薬学会の論文
• 1981-05-23

著者

• 山下 春雄

  Department of Chemistry, Research Laboratories, Teikoku Hormone Mfg. Co., Ltd.,

• 権藤 昌昭

  Research Department, Teikoku Hormone Mfg. Co., Ltd.,

• 榊原 恭一

  Research Department, Teikoku Hormone Mfg. Co., Ltd.,

• 真仁田 英明

  Chemical and Pharmacological Research Laboratories, Teikoku Hormone Mfg. Co.,Ltd.

• 山下 春雄

  Department Of Chemistry Research Laboratories Teikoku Hormone Mfg. Co. Ltd.

• 榊原 恭一

  Research Department Teikoku Hormone Mfg. Co. Ltd.

• 権藤 昌昭

  Research Department Teikoku Hormone Mfg. Co. Ltd.

• Gondo M

  Research Department Teikoku Hormone Mfg. Co. Ltd.

• 権藤 昌昭

  Chemical Research Laboratories, Teikoku Hormone Mfg. Co., Ltd.

• 山下 春雄

  Chemical Research Laboratories, Teikoku Hormone Mfg. Co., Ltd.

• 榊原 恭一

  Chemical Research Laboratories, Teikoku Hormone Mfg. Co., Ltd.

• 真仁田 英明

  Chemical Research Laboratories Teikoku Hormone Mfg. Co. Ltd.

• Sakakibara K

  Research Department Teikoku Hormone Mfg. Co. Ltd.

関連論文

• Synthesis of 4-(Methoxyethyl) Monobactams by a Chemicoenzymatic Approach
• Synthetic Study of cis-3-Amino-4-(1-hydroxyalkyl)azetidin-2-ones Using L-Aspartic Acid as a Chiral Synthon
• Synthetic Studies on an Antitumor Antibiotic, Bleomycin. XII. Preparation of an L-2,3-Diaminopropionic Acid Derivative as a Synthetic Intermediate
• Synthesis and Antitumor Activity of Novel Dolastatin 10 Analogs
• Extraction and Purification of Radioprotective Substance from Calf Thymus
• Studies on Immunological Assay of Urinary Estrogens. IV. Comparison of the Latex Agglutination Inhibition Reaction Method with Gas Chromatography-Mass Spectrometry
• Studies on Immunological Assay of Urinary Estrogens. III. A New Latex Agglutination Inhibition Reaction Method

スポンサーリンク