3-[4-(Bromomethyl)phenyl]-7-(diethylamino)-2H-1-benzopyran-2-one (MPAC-Br) : A Highly Sensitive Fluorescent Derivtization Reagent for Carboxylic Acids in High-Performance Liquid Chromatography
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概要
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7-Dimethylaminocoumarin derivatives were synthesized and the characteristics of their absorption and emission spectra were investigated. Among them, 3-aryl-7-dimethylaminocoumarins were shown to be promising candidates as the fluorogenic groups for highly sensitive derivatization reagents. Based on the spectroscopic studies, 3-[4-(bromomethyl)phenyl]-7-(diethylamino)-2H-1-benzopyran-2-one (MPAC-Br) was designed and synthesized as a fluorescent derivatization reagent for carboxylic acids in HPLC. Saturated carboxylic acids (C_4-C_<18>) were derivatized almost quantitatively into the corresponding fluorescent MPAC-esters by treatment with MPTAC-Br. The MPAC-esters of these acids were clearly separated on a reversed-phase HPLC column (Intertsil ODS-2,mobile phase : methanol-water, excitation wavelength 403 nm; emission wavelength 474 nm). The detection limit (S/N=17) of lauric acid, as a test compound, was 15 fmol/10 μl.
- 公益社団法人日本薬学会の論文
- 1996-04-15
著者
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Machida Minoru
Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Kamada S
Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Takechi Haruko
Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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KAMADA Seiji
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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