Spectrofluorimetric Determination of 10^<-7> M Levels of Thiol Compounds Using Silver(I)-5,10,15,20-Tetrakis(4-sulfophenyl)porphine Complex

概要

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5,10,15,20-Tetrakis(4-sulfophenyl)porphine (TPPS) has a strong fluorescence, while the silver(I) complex [Ag(I)_2-TPPS] has very little fluorescence. Also, the Ag(I)_2-TPPS complex easily releases the silver ion from the porphine ring in the presence of thiol compounds such as L-cysteine, glutathione and 2-mercaptoethanol. Based on these results, a highly sensitive spectrofluorimetric determination of 10^<-7> M levels of thiol compounds was developed. Calibration curves were linear in the concentration ranges of 0-5×10^<-7> M for L-cysteine, 0-5×10^<-7>M for glutathione and 0-2×10^<-6> M for 2-mercaptoethanol. Detection limits (3σ) were 1×10^<-10> M for L-cysteine, 5×10^<-11> M for glutathione and 2×10^<-10> M for 2-mercaptoethanol. Precisions were 1.89% for L-cysteine (2.5×10^<-7> M), 3.33% for glutathione (2.5×10^<-7> M) and 2.33% for 2-mercaptoethanol (1.0×10^<-6> M).
公益社団法人日本薬学会の論文
1996-01-15