Spectrophotometric Determination of Peroxidase by the Oxidarive Decomposition of Copper-Phthalocyanine Complex using Peroxomonosulfate

概要

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Peroxidase (POD) was found to act as an excellent catalyst during the oxidative decomposition reaction of the copper-phthalocyanine complex (Cu-pts) using peroxomonosulfate. POD also decomposed with Cu-pts during the reaction. Therefore, the simultaneous decomposition of indicator and catalyst provides a characteristic L-shape absorbance-time curve. Based on these findings, an indicator system of POD, which has a slight variation in the measured absorbance for time and high precision, was established. This spectrophotometry was able to make determination over the range of 0.1 to 30 μg/ml of POD. The detection limits (3σ) were 0.02 μg/ml and the relative standard deviation was 3.7% for 0.5 μg/ml of POD (10 determinations) The proposed method has been applied to the determination of human interleukin-8 which was added to human serum using POD as the label in an immunoassay.
公益社団法人日本薬学会の論文
1998-12-15