Metabolic Difference between 3,4-Dihydroxyphenylpyruvic Acid (DHPP) and 3-Methoxy-4-hydroxyphenylpyruvic Acid (MHPP)
スポンサーリンク
概要
- 論文の詳細を見る
3,4-Dihydroxyphenylpyruvic acid (DHPP) was mainly metabolized to L-3,4-dihydroxyphenylalanine (L-DOPA) through transamination, while 3-methoxy-4-hydroxy-phenylpyruvic acid (MHPP) was mainly metabolized to 3-methoxy-4-hydroxyphenyllactic acid (MHPL) through reduction. In order to explain the metabolic difference between these two aromatic α-keto acids related with DOPA, the metabolism of ^<14>C-labeled DHPP and MHPP was compared in vitro. There was no significant difference between the two α-keto acids in the initial rate of transamination to the corresponding amino acids using rat liver mitochondrial fraction. The K_m values to mitochondrial transaminase were calculated to be 8 mM and 6 mM for DHPP and MHPP, respectively. NADH-dependent activity for reducing DHPP and MHPP to the corresponding aromatic α-hydroxy acids was the highest in the heart followed by the kidney, muscle and liver and was not inhibited with oxamate (5 mM), the inhibitor of lactate dehydrogenase, indicating that aromatic α-keto acid reductase (AKAR) predominantly participates in this reaction. The initial rate of DHPP reduction with purified rat liver AKAR preparation was, however, only about 1/10 of that of MHPP reduction. The K_m value of DHPP (3.6 mM) was about two-fold larger than that of MHPP (1.5 mM), demonstrating a lower affinity of DHPP to AKAR than that of MHPP.
- 社団法人日本薬学会の論文
- 1978-07-25
著者
-
進藤 英世
Central Research Laboratories, Sankyo Co., Ltd.
-
前田 敏彦
Central Research Laboratories Sankyo Co. Ltd.
-
進藤 英世
Central Research Laboratories Sankyo Co. Ltd.
-
進藤 英世
Central Research Labolatories, Sankyo Go., Ltd.
関連論文
- Transport of Organic Compounds through Biological Membranes. II. Red Cell Permeability to O-Acyl-S-benzoylthiamines
- Transport of Organic Compounds through Biological Membranes.I.Accumulative Uptake of S-Benzoylthiamine by Human Erythrocytes
- Determination of Pancuronium Bromide and Its Metabolites in Human Urine by Dye-Extraction Method-Relation between the Extractability and Structure of Quaternary Ammonium Ions
- Studies on the Metabolism of D- and L-Isomers of 3,4-Dihydroxy-phenylalanine (DOPA). IV.^ Urinary and Tissue Metabolites of D- and L-DOPA-^C after Intravenous and Oral Administration to Rats
- Studies on the Metabolism of D- and L-Isomers of 3,4-Dihydroxy-phenylalanine (DOPA). III. Absorption, Distribution and Excretion of D- and L-DOPA-^C in Rats following Intravenous and Oral Administration
- Studies on the Metabolism of Oxazolam. III. Absorption from Rat Intestine and Metabolites in the Liver, Brain and Blood
- Urinary Metabolites of 1-(3-Mercapto-2-D-methyl-1-oxopropyl)-L-proline (SQ-14225), a New Antihypertensive Agent, in Rats and Dogs
- Autoradiographic Studies on the Distribution of Quaternary Ammonium Compounds. IV. Adsorption of Bisonium Ions to Rat Tissues as revealed by in Vitro Whole-body Adsorption Autoradiography
- Mechanism of Intestinal Absorption and Brain Uptake of L-5-Hydroxytryptophan in Rats, as compared to Those of L-3,4-Dihydroxyphenylalanine
- Studies on the Metabolism of D- and L-Isomers of 3,4-Dihydroxyphenylalanine (DOPA). V. Mechanism of Intestinal Absorption of D- and L-DOPA-^C in Rats
- Metabolic Fate of 3,4-Dihydroxyphenylpyruvic Acid (DHPP) in Rats. II. In Vivo Evaluation of DHPP as a Possible New Precursor of Brain Dopamine
- Whole-body Autoradiographic Studies on the Distribution of ^C-Labeled D-and L-5-Hydroxytryptophan, 5-Hydroxytryptamine and 5-Hydroxyindole-3-acetic Acid in Rats
- Autoradiographic Studies on the Distribution of Quaternary Ammonium Compounds. III. Distribution, Excretion and Metabolism of ^C-Labeled Pancuronium Bromide in Rats
- Studies on the Metabolism of D- and L-Isomers of 3,4-Dihydroxyphenylalanine (DOPA). II. Autoradiographic Study on the Distribution of ^C-Labeled D- and L-DOPA after Oral Administration in Rats
- Studies on the Metabolism of D- and L-Isomers of 3,4-Dihydroxyphenylalanine (DOPA). I. Autoradiographic Study on the Distribution of ^C-Labeled D-and L-DOPA and Dopamine after Intravenous Administration in Rats
- Bumetanideのガスクロマトグラフィ-による定量と健康人における尿中排泄〔英文〕
- Metabolic Difference between 3,4-Dihydroxyphenylpyruvic Acid (DHPP) and 3-Methoxy-4-hydroxyphenylpyruvic Acid (MHPP)
- Metabolic Fate of 3,4-Dihydroxyphenylpyruvic Acid (DHPP) in Rats. I. Specific Transformation of DHPP to L-3,4-Dihydroxy-phenylalanine in Vitro
- Metabolic Pathway of L-3-Methoxy, 4-hydroxyphenylalanine (3-O-MethylDOPA)-Participation of Tyrosine Aminotransferase and Lactate Dehydrogenase
- Autoradiographic Studies on the Distribution of Drugs. I. Distribution of ^C-Anisotropine Methbromide in Mice
- Studies on the Metabolism of Oxazolam. I. Distribution and Excretion Studies
- Studies on the Metabolism of D-and L-Isomers of 3,4-Dihydroxyphenylalanine (DOPA). VI. Metabolism of D-DOPA in Rat Kidney
- Autoradiographic Studies on the Distribution of Quaternary Ammonium Compounds. II. Distribution of ^14C-Labeled Decamethonium, Hexamethonium and Dimethonium in Mice
- Metabolism of Cloxazolam. I. Distribution, Excretion and Biotransformation in Rats and Mice
- STRUCTURAL SPECIFICITIES FOR THE ACTIVE TRANSPORT SYSTEM OF THIAMINE IN RAT SMALL INTESTINE
- Metabolic Fate and Mechanism of Action of Chloroethylthiamine : VI. Mechanism of Anticoccidial Action of Chloroethylthiamine
- Metabolic Fate and Mechanism of Action of Chloroethylthiamine:III. Active Transport of Thiamine from Chick Intestine and Competitive Inhibition by Chloroethylthiamine
- Metabolic Fate and Mechanism of Action of Chioroethyithiamine:II. Movement and Fate of Chloroethylthiamine along Intestinal Lumen after Oral Administration to Chick
- Metabolic Fate and Mechanism of Action of Chloroethylthiamine:V. Mechanism of Formation of Thiamine and Other Metabolites in Chick
- ACTIVE TRANSPORT OF THIAMINE FROM RAT SMALL INTESTINE
- Metabolic Fate and Mechanism of Action of Chloroethylthiamine:I. Absorption of Chloroethylthiamine from Chick Intestine and Its Competition with Thiamine
- TRANSPORT OF THIAMINE INTO RED BLOOD CELLS OF THE RAT
- Metabolic Fate and Mechanism of Action of Chloroethylthiamine:IV. Urinary Metabolites of 35S-Chloroethylthiamine in Chicks