Colorimetric Determination of Carboxylic Acids with 2-Nitrophenylhydrazine Hydrochloride
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Aliphatic and aromatic acids and 2-nitrophenylhydrazine hydrochloride were coupled in aqueous ethanol with dicyclohexylcarbodiimide as a coupling agent in the presence of pyridine and thiourea. The resultant hydrazide solution was made alkaline to develop a violet color, and heated at 60°to decolorize the brown blank color. This colorimetric method of determination is suitable for aqueous and aqueous ethanol samples of carboxylic acids.
- 1978-05-25
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