Fluorescence and Structure of Proteins as measured by Incorporation of Fluorophore. III. Fluorescence Characteristics of N-[p-(2-Benzoxazolyl) phenyl] maleimide and the Derivatives

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概要

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• Among fluorescent-labeled sulfhydryl reagents hitherto synthesized in this laboratory, N-[p-(2-benzoxazolyl) phenyl] maleimide (I) was selected as a typical example and its fluorescent characteristics were determined. In contrast to that I shows no substantial fluorescence, its cysteine ester-adduct (II) exhibits strong fluorescence with emission maxima at 352 and 365 mμ. This spectroscopic behavior of this type of reagent may be favorably utilized in the application to proteins. Since N-[p-(2-benzoxazolyl) phenyl] succinimide (III) is regarded as the simplest model compound of the adducts of I with sulfhydryl substrates, fluorescent spectra of III in various media were examined. The absorption spectra of III were also systematically examined by comparing spectra of IV, VII, VIII and III. General procedures of fluorescence determination were described and interpretation of the spectra was discussed.

• 公益社団法人日本薬学会の論文
• 1968-09-25

著者

• 関根 隆光

  School of Medicine, Juntendo University

• 町田 実

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 町田 実

  Formulation Technology Lab. Development & Technology Div. Chugai Pharmaceutical Co. Ltd.

• 国分 ■

  Research Institute of Applied Electricity, Hokkaido University

• 国分 ■

  Research Institute Of Applied Electricity Hokkaido University

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