Application of Chromatography. XXXII. Biosynthesis of Riboflavin by Eremothecium ashbyii.
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It was ascertained by paper partition chromatography, ultraviolet spectrum, and bioassay with L. casei that the green fluorescent substance, G compound, isolated from the mycelium of Er. ashbyii readily reacts with diacetyl or with acetoin to form riboflavin. These results established the structure 8-ribityl-6,7-dimethyllumazine, formerly proposed by the author for the G compound. On the other hand, a large quantity of acetoin was detected in the mycelium and the culture filtrate, and comparing the amount with those of riboflavin and G compound in these materials, the assumption was made that the biosynthesis of riboflavin by Er. ashbyii might be effected by condensation of acetoin with G compound at the position of the methyl groups.
- 公益社団法人日本薬学会の論文
- 1957-04-20
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