Hyaluronate Depolymerization Following Thermal Decomposition of Oxytetracycline
スポンサーリンク
概要
- 論文の詳細を見る
Depolymerization of sodium hyaluronate(HA)by tetracyclines was investigated. Reduction in HA molecular weight was followed by size exclusion chromatography with a low angle laser light scattering detector. On mixing with oxytetracycline hydrochloride(OTC)solution and incubating at 37℃, HA was gradually depolymerized. OTC, a representative antibiotic, is known as a photosensitizer, and phototoxic side effects relevant to radicals have been reported. However, HA depolymerization required no irradiation. As time passed, OTC solution incubated at 37℃ got colored reddish brown, even in the dark. With reversed-phase HPLC separation, several peaks derived from decomposed OTC appeared. One of the peaks had an absorbance in the visible range. A quantitative correlation between the discoloration and the HA depolymerization rate was obtained. On the other hand, when samples were incubated below 25℃, change of color was slight, and practically no HA depolymerization was observed after up to 4h. Oxygen depletion by nitrogen saturation or addition of mannitol also prevented the depolymerization. Under anaerobic conditions, the color of the solution did not change, whereas it turned red under aerobic conditions in the presence of mannitol. The mannitol did not inhibit the OTC decomposition, but it preserved HA from damage. On the basis of the known decomposition of OTC and the results of HPLC separation, anhydrooxytetracycline can be proposed as the derivative causing HA depolymerization.
- 公益社団法人日本薬学会の論文
- 2001-01-01
著者
-
Okada S
National Institute Of Health Sciences
-
MIYAZAKI Tamaki
National Institute of Health Sciences
-
YOMOTA Chikako
National Institute of Health Sciences
-
OKADA Satoshi
National Institute of Health Sciences
関連論文
- Hyaluronate Depolymerization Following Thermal Decomposition of Oxytetracycline
- IC/ICP-MSによるパン中臭素酸カリウムの特異的分析法
- Miscibility of Nifedipine and Hydrophilic Polymers as Measured by ^1H-NMR Spin-Lattice Relaxation
- Feasibility of ^F-NMR for Assessing the Molecular Mobility of Flufenamic Acid in Solid Dispersions
- Physical Stability of Amorphous Acetanilide Derivatives Improved by Polymer Excipients
- Characteristics of Hyaluronate-hydroxyethyl Acrylate Blend Gel and Release of Cationic Amphiphilic Solutes
- Preparation and Characterization of Hyaluronate-Hydroxyethyl Acrylate Blend Hydrogel for Controlled Release Device
- Prediction of Measurement Precision by Chemometric Invalidation of Flow Pulsation in Refractive Index Detection of Liquid Chromatography
- Discrimination limit for purity test of human insulin by capillary electrophoresis
- Inhibition of Mannitol Crystallization in Frozen Solutions by Sodium Phosphates and Citrates
- Freeze-Drying of Proteins in Glass Solids Formed by Basic Amino Acids and Dicarboxylic Acids
- Stabilization of Protein Structure in Freeze-Dried Amorphous Organic Acid Buffer Salts
- Glass-State Amorphous Salt Solids Formed by Freeze-Drying of Amines and Hydroxy Carboxylic Acids : Effect of Hydrogen-Bonding and Electrostatic Interactions
- Sustained Release of Flufenamic Acid from a Drug-Triacetyl-β-Cyclodextrin Complex
- EFFECT OF CHOLESTEROL ON THE FORMATION OF INTERDIGITATED GEL PHASE IN LECITHIN MEMBRANES
- Comparison of Particle Size and Dispersion State among Commercial Cyclosporine Formulations and Their Effects on Pharmacokinetics in Rats
- Alterations in the Detergent-Induced Membrane Permeability and Solubilization of Saturated Phosphatidylcholine/Cholesterol Liposomes: Effects of Poly(ethylene glycol)-Conjugated Lipid
- Polyethylene Glycol Prevents in Vitro Aggregation of Slightly Negatively-Charged Liposomes Induced by Heparin in the Presence of Bivalent Ions
- Component Crystallization and Physical Collapse during Freeze-Drying of L-Arginine–Citric Acid Mixtures
- Freeze-Drying of Proteins in Glass Solids Formed by Basic Amino Acids and Dicarboxylic Acids
- Thermotropic Phase Behavior of Hydrogenated Soybean Phosphatidylcholine–Cholesterol Binary Liposome Membrane