Effect of Brain on Proliferative Activity of Adenohypophysial Primordial Cells in vitro(Endocrinology)

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The pattern of cell proliferation in the fetal rat adenohypophysial primordium was investigated both in vivo and in vitro with special reference to the diencephalic floor. Proliferating cells were labelled by bromodeoxyuridine (BrDU) followed by its immunohistochemical detection with a monoclonal antibody. In fetal rats of 12.5 days of age, BrDU-labelled cells were distributed almost evenly throughout the adenohypophysial primordium. On days 13.5 and 14.5, on the other hand, labelling was mostly confined to the dorsal wall of the adenohypophysial primordium that was in contact with the diencephalic floor. To examine the in vitro effect of the developing diencephalic floor on cell proliferation of the adenohypophysial primordium, Rathke's pouches were isolated from fetal rats on days 12.5 and 13.5 of gestation and kept in organ culture for 1-2 days with or without the brain. The incidence of BrDU-labelled cells was markedly high if the diencephalic floor was left intact. Moreover, proliferating adenohypophysial primordial cells were concentrated in the region adjacent to brain tissue. In the absence of brain, only a small number of cells were labelled. Such a low incidence of labelling was also observed when brain was replaced by liver. From these results we conclude that the developing diencephalon is essential for proliferation of adenohypophysial primordial cells. It remains to be settled if the adoral part of primordial cells can also respond to this neural agent.
社団法人日本動物学会の論文
1992-06-15

Effect of Brain on Proliferative Activity of Adenohypophysial Primordial Cells in vitro(Endocrinology)

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