In vitro Evidence for a Neural Factor(s) Involved in the Proliferation of Adenohypophysial Primordial Cells in Fetal Rats

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Our previous in vitro data have shown that proliferative activity of adenohypophysial primordial cells of the rat drops markedly after removal of the developing diencephalic floor. In this study, we transplanted the diencephalic floor to an area of the adenohypophysial primordium that had originally not been in contact with the brain. This transplantation experiment was carried out on day 13.5 of fetal age, when most of the proliferating adenohypophysial cells are directed toward the developing brain. DNA-replicating cells in the culture explants were revealed one day later by incorporation of bromodeoxyuridine (BrDU) followed by its detection by the use of a monoclonal antibody. The results of the transplantation experiments differed, depending on which part of the primordium came in contact with the neural tissue. If the diencephalic floor was attached to the dorsal half of the adenohypophysial primordium, a new brain-dependent pattern of cell proliferation took place in the explants. Transplantation of the diencephalic floor, on the other hand, to the ventral half of the developing adenohypophysis resulted in a decrease in the proliferative rate of the adenohypophysial cells. The cell proliferative activity of brain-deprived adenohypophysial explants was very low. Thus the results of our study show that the presumptive neural factor(s) contained in the developing brain affects only dorsally located cells of the adenohypophysial primordium.
社団法人日本動物学会の論文
1993-12-15

In vitro Evidence for a Neural Factor(s) Involved in the Proliferation of Adenohypophysial Primordial Cells in Fetal Rats

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