Classification and Characterization of Ten Hemocytes Types in the Tunicate Halocynthia roretzi(Immunology)

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We propose a standard condition for classifying living hemocytes of Halocynthia roretzi: adhering or spreading on glass in the presence of EGTA at pH 6.0 at room temperature (adhering cells) Accoring to morphological characteristics revealed by vital staining, autonomous fluorescence and an effect of NH_4 ions, we classified hemocytes into ten groups; phagocytes type 1 and 2 (pi- and p2-cells), granular cells type 1, 2, and 3 (gl-, g2- and g3-cells), vacuolated cells type 1, 2, 3 and 4 (vl-, v2s v3-, and v4-cells) and lymphoid cells (ly-cells). Two types of phagocytes, pi- and p2-cells, were the only cells which actively phagocytize and spread as adhering cells. They were spherical cells with a diameter of 20-40μm and 40-50μm fan before spreading. Three types of small-granule containing cells and four types of large-vacuole containing cells were also clearly distinguished not only by their size but also by their behavior or properties after vital staining. Ly-cells were small and spherical and have been referred to as lymphocytes or hemoblasts. We correlated seven groups (pi-, p2-, gl-, g2-, v3-, v4- and ly-cells) with the former classification, and identified for the first time three types of hemocytes (g3-, vl- and v2-cells). The relative proportions of various hemocyte types did not agree with earlier reports, and pi-cells, accounting for 30-45%, were found to be the largest group. These basic analyses of cell types are essential for future studies of immunodefense properties.
社団法人日本動物学会の論文
1991-10-15

Classification and Characterization of Ten Hemocytes Types in the Tunicate Halocynthia roretzi(Immunology)

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