Expression Pattern and Transcriptional Control of SoxB1 in Embryos of the Ascidian Halocynthia roretzi(Developmental Biology)
スポンサーリンク
概要
- 論文の詳細を見る
The Sox family is a large group of transcription factors that are characterized by the presence of a DMA-binding HMG domain. We isolated HrSoxB1, an ascidian homolog of the Sox gene that belongs to the B1 subclass of the Sox family, from Halocynthia roretzi. Expression was initiated as early as the 8-cell stage. During cleavage stages, HrSoxB1 was expressed in three quarters of embryonic blastomeres but not in posterior-vegetal (B-line) blastomeres. Misexpression of mRNAs of HrPEM but not of macho-1, whose maternal mRNAs are localized to the posterior-vegetal cytoplasm of eggs and early embryos, repressed the anterior-vegetal expression of HrSoxB1. This result suggests that the zygotic expression of HrSoxB1 is controlled by the localized maternal mRNA. When HrSoxB1 was overexpressed in early embryos, ectopic expression of HrBra, a gene for a transcription factor expressed in notochord blastomeres, occurred in the most posterior blastomeres (B7.5), although these blastomeres did not eventually differentiate into notochord but developed into muscle, as they do in normal embryogenesis. In later embryogenesis, HrSoxB1 was specifically expressed in neural plate cells. However, overexpression of HrSoxB1 did not affect the expression of a neural plate marker gene, HrETR-1.
- 社団法人日本動物学会の論文
- 2003-01-25
著者
-
Nishida Hiroki
Department Of Biological Science Graduate School Of Science Osaka University
-
Miya Takahito
Department Of Biological Sciences Tokyo Institute Of Technology
関連論文
- FUNCTION OF MACHO-1 AS THE PVC FACTOR : THE FATE DETERMINATION MECHANISM IN THE VEGETAL HEMISPHERE OF ASCIDIAN EMBRYOS(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- MATERAL mRNAs THAT ARE LOCALIZED TO MYOPLASM IN ASCIDIAN EGG ASSOCIATE WITH CORTIAL ENDOPLASMIC RETICULUM(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- EARLY CLEAVAGE PATTERN OF THE LARVACEAN, OIKOPLEURA DIOICA(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- ROLE OF BETA-CATENIN SIGNALING IN HALOCYNTHIA RORETZI(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- A SENSITIVE TRANSCRIPTOME ANALYSIS OF DIVERGENCE OF THE EMBRYONIC EPIDERMAL AND NEURAL FATES OF THE ASICIDIAN HALOCYNTHIA RORETZI(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- SUPPRESSION OF MACHO-1 FUNCTION BY FGF AND BMP SIGNALING IS REQUIRED FOR THE POSTERIOR-ENDODERM FORMATION IN ASCIDIAN EMBRYOS(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Localization of mitochondrial large ribosomal RNA in the myoplasm of the early ascidian embryo
- Ultrastructural studies on the centrosome-attracting body : Electron-dense matrix and its role in unequal cleavages in ascidian embryos
- MOLECULAR MECHANISM OF LOSS OF THE COMPETENCE IN ASCIDIAN NOTOCHORD INDUCTION(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Duration of competence and inducing capacity of blastomeres in notochord induction during ascidian embryogenesis
- Centrosome-attracting body : A novel structure closely related to unequal cleavages in the ascidian embryo
- Polo-like kinase 1 is required for localization of Posterior End Mark protein to the centrosome-attracting body and unequal cleavages in ascidian embryos
- LOCALIZATION OF β-CATENIN PROTEIN IN EARLY EMBRYOGENESIS OF ASCIDIAN HALOCYNTHIA RORETZI(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Analysis of the temporal expression of endoderm-specific alkaline phosphatase during development of the ascidian Halocynthia roretzi
- A Large-Scale Whole-Mount in situ Hybridization System: Rapid One-Tube Preparation of DIG-Labeled RNA Probes and High Throughput Hybridization using 96-Well Silent Screen Plates
- DESCRIPTION OF EARLY CLEAVAGE PATTERN OF THE LARVACEAN, OIKOPLEURA DIOICA(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Failure of Muscle Myosin Heavy-Chain Gene Expression in Quarter Ascidian Embryos Developed from the Secondary Muscle Lineage Cells(Developmental Biology)
- Repression of Zygotic Gene Expression in the Putative Germline Cells in Ascidian Embryos(Developmental Biology)
- CELL LINEAGES IN LARVAL BRAIN FORMATION DURING ASCIDIAN EMBRYOGENESIS(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Development of the appendicularian Oikopleura dioica : Culture, genome, and cell lineages
- Expression Pattern and Transcriptional Control of SoxB1 in Embryos of the Ascidian Halocynthia roretzi(Developmental Biology)
- Monoclonal Antibodies against Differentiating Mesenchyme Cells in Larvae of the Ascidian Halocynthia roretzi
- Role of the FGF and MEK signaling pathway in the ascidian embryo
- Developmental Roles of Nuclear Complex Factors Released during Oocyte Maturation in the Ascidians Halocynthia roretzi and Boltenia villosa
- Spatio-temporal pattern of MAP kinase activation in embryos of the ascidian Halocynthia roretzi
- WNT-5 IS CELL-AUTONOMOUSLY INVOLVED IN CONVERGENT EXTENSION MOVEMENT OF NOTOCHORD CELLS IN THE ASCIDIANS, HALOCYNTHIA RORETZI(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Analysis of Cell Fate Specification during Ascidian Early Embryogenesis(The Zoological Society Prize Winner,PROCEEDING OF THE 74^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)