Developmental Roles of Nuclear Complex Factors Released during Oocyte Maturation in the Ascidians Halocynthia roretzi and Boltenia villosa
スポンサーリンク
概要
- 論文の詳細を見る
The developmental roles of factors associated with the nuclear complex of Halocynthia roretzi and Boltenia villosa oocytes were investigated by cutting mature oocytes into animal and vegetal merogons before and during GVBD. Animal and vegetal merogons were cultured in sea water until the GV cytoplasm had dispersed within the cytoplasm of control oocytes and then they were cross-fertilized and scored for their ablility to undergo normal development. Halocynthia oocyte fragments produced from the animal region of oocytes containing intact GVs exhibited a low frequency of polyspermy, a high frequency of fertilization and cleavage, and a high frequency of expressing an epidermal antigen, Epi-2. In contrast, merogons produced from the vegetal region of Halocynthia oocytes in which GVs were intact exhibited a high frequency of polyspermy, did not undergo cell division, and expressed a high frequency of Epi-2 expression. When vegetal fragments were produced after the dispersal of approximately 50-70% of the GV nucleoplasm, these merogons exhibited a low frequency of polyspermy, high frequencies of cell division(including the formation of epidermal layer), and in most cases expressed Epi-2. Vegetal Boltenia fragments produced during GVBD in some cases developed into larvae. These results suggest that the ascidian GV nucleoplasm may contain factors required for fertilization and cell division and that epidermal determinants reside in the oocyte cytoplasm.
- 社団法人日本動物学会の論文
著者
-
Nishida Hiroki
Department Of Biological Science Graduate School Of Science Osaka University
-
Bates William
Bamfield Marine Station, the Department of Biology, University of Victoria
-
Bates William
Bamfield Marine Station The Department Of Biology University Of Victoria : Department Of Life Scienc
-
Bates William
Bamfield Marine Station Bamfield British Columbia Vor 1bo And The Department Of Biology University O
関連論文
- FUNCTION OF MACHO-1 AS THE PVC FACTOR : THE FATE DETERMINATION MECHANISM IN THE VEGETAL HEMISPHERE OF ASCIDIAN EMBRYOS(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- MATERAL mRNAs THAT ARE LOCALIZED TO MYOPLASM IN ASCIDIAN EGG ASSOCIATE WITH CORTIAL ENDOPLASMIC RETICULUM(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- EARLY CLEAVAGE PATTERN OF THE LARVACEAN, OIKOPLEURA DIOICA(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- ROLE OF BETA-CATENIN SIGNALING IN HALOCYNTHIA RORETZI(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- A SENSITIVE TRANSCRIPTOME ANALYSIS OF DIVERGENCE OF THE EMBRYONIC EPIDERMAL AND NEURAL FATES OF THE ASICIDIAN HALOCYNTHIA RORETZI(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- SUPPRESSION OF MACHO-1 FUNCTION BY FGF AND BMP SIGNALING IS REQUIRED FOR THE POSTERIOR-ENDODERM FORMATION IN ASCIDIAN EMBRYOS(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Localization of mitochondrial large ribosomal RNA in the myoplasm of the early ascidian embryo
- Ultrastructural studies on the centrosome-attracting body : Electron-dense matrix and its role in unequal cleavages in ascidian embryos
- MOLECULAR MECHANISM OF LOSS OF THE COMPETENCE IN ASCIDIAN NOTOCHORD INDUCTION(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Duration of competence and inducing capacity of blastomeres in notochord induction during ascidian embryogenesis
- Centrosome-attracting body : A novel structure closely related to unequal cleavages in the ascidian embryo
- Polo-like kinase 1 is required for localization of Posterior End Mark protein to the centrosome-attracting body and unequal cleavages in ascidian embryos
- LOCALIZATION OF β-CATENIN PROTEIN IN EARLY EMBRYOGENESIS OF ASCIDIAN HALOCYNTHIA RORETZI(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Analysis of the temporal expression of endoderm-specific alkaline phosphatase during development of the ascidian Halocynthia roretzi
- A Large-Scale Whole-Mount in situ Hybridization System: Rapid One-Tube Preparation of DIG-Labeled RNA Probes and High Throughput Hybridization using 96-Well Silent Screen Plates
- DESCRIPTION OF EARLY CLEAVAGE PATTERN OF THE LARVACEAN, OIKOPLEURA DIOICA(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Failure of Muscle Myosin Heavy-Chain Gene Expression in Quarter Ascidian Embryos Developed from the Secondary Muscle Lineage Cells(Developmental Biology)
- Repression of Zygotic Gene Expression in the Putative Germline Cells in Ascidian Embryos(Developmental Biology)
- CELL LINEAGES IN LARVAL BRAIN FORMATION DURING ASCIDIAN EMBRYOGENESIS(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Development of the appendicularian Oikopleura dioica : Culture, genome, and cell lineages
- Expression Pattern and Transcriptional Control of SoxB1 in Embryos of the Ascidian Halocynthia roretzi(Developmental Biology)
- Monoclonal Antibodies against Differentiating Mesenchyme Cells in Larvae of the Ascidian Halocynthia roretzi
- Role of the FGF and MEK signaling pathway in the ascidian embryo
- Developmental Roles of Nuclear Complex Factors Released during Oocyte Maturation in the Ascidians Halocynthia roretzi and Boltenia villosa
- Spatio-temporal pattern of MAP kinase activation in embryos of the ascidian Halocynthia roretzi
- Localization of constitutive heat shock proteins in developing ascidians
- WNT-5 IS CELL-AUTONOMOUSLY INVOLVED IN CONVERGENT EXTENSION MOVEMENT OF NOTOCHORD CELLS IN THE ASCIDIANS, HALOCYNTHIA RORETZI(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Analysis of Cell Fate Specification during Ascidian Early Embryogenesis(The Zoological Society Prize Winner,PROCEEDING OF THE 74^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)