Monoclonal Antibodies against Differentiating Mesenchyme Cells in Larvae of the Ascidian Halocynthia roretzi
スポンサーリンク
概要
- 論文の詳細を見る
Mechanisms of cell specification of mesenchyme during ascidian embryogenesis are poorly understood. This is because no good molecular markers have been available to evaluate differentiation of the mesenchyme cells. To obtain molecular markers of mesenchyme differentiation, we established monoclonal antibodies, Mch-1 and Mch-3, that recognize antigens present in the mesenchyme cells of the larva of Halocynthia roretzi. The antigens recognized by both antibodies start to be detectable in the mesenchyme cells at the late tailbud stage. The Mch-3 antibody specifically recognized all mesenchyme cells of the larva, whereas the Mch-1 antibody stained the cells only in the anterior portions of mesenchyme clusters in the trunk region of the larva. The Mch-1 antibody also stained trunk lateral cells, In addition, both antibodies recognized the mesenchyme cells in the ventro-lateral boundary between endoderm and epidermis that are migrating to the anterior head region of the larva. The partial embryos that originated from the mesenchymelineage cells at the 8-cell stage expressed the Mch-1 and Mch-3 antigens. The Mch-1 and Mch-3 antibodies will be useful as immunological probes for studying the specification mechanisms of mesenchyme cells.
- 社団法人日本動物学会の論文
著者
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Nishida Hiroki
Department Of Biological Science Graduate School Of Science Osaka University
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Kim G
Department Of Life Science Tokyo Institute Of Technology Nagatsuta
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Kim Gil
Department of Life Science, Tokyo Institute of Technology, Nagatsuta
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Kim Gil
Department Of Biological Sciences Tokyo Institute Of Technology
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Nishida Hiroki
Department Of Life Science Tokyo Institute Of Technology Nagatsuta
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Kim G.J.
Department of Life Science, Tokyo Institute of Technology, Nagatsuta
関連論文
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