Effects of the Radical Scavenger, Water Soluble Protein from Broad Beans on Lipofuscin, Cathepsin B, Cell Growth in Human Lung Fibroblasts(Cell Biology)

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概要

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• We purified the free-radical scavenger "water soluble protein (WSP)" from broad beans. Oxidative stress, as well as diminished activity of lysosomal proteolytic enzymes, are known to induce lipofuscin accumulation in a variety of cell types. In order to clarify the roles of oxidative stress and lysosomal proteolysis in lipofuscin accumulation, human lung fibroblasts were treated with WSP. Hydrocortisone (HC) which is known to inhibit superoxide generation, was compared with WSP. Cathepsin B activity in young cells showed a decrease during cell culture from 0.203±0.013 mU/μg protein (4 weeks) to 0.067±0.018 (6 weeks) with an unparalleled increase in lipofuscin (as autofluorescence intensity, AFI/μg protein) from 1.46±0.05 (4 weeks) to 1.55±0.04 (6 weeks). Similarly, the activity in old cells exhibited a decrease from 0.208+0.013 mU/μg protein (4 weeks) to 0.043±0.005 (6 weeks) with an unparalleled increase in lipofuscin from 2.11 ±0.06 AFI/μg protein (4 weeks) to 2.27±0.05 (6 weeks). Young cells treated with WSP (1.25μg/ml) for 6 weeks showed increased cathepsin B activity (0.118±0.009 mU/ μg protein) as compared to the control, but the activity in WSP treatment for 4 weeks decreased by 89%. Young cells treated with HC (5μg/ml) were equal in cathepsin B activity to the control. On the other hand, old cells treated with WSP for 4 weeks exhibited the decreased activity (0.179±0.008 mU/μg protein). In contrast, HC treatment for 4 weeks increased the activity (36%). WSP treatment for 6 weeks was effective in reducing lipofuscin accumulation in young (25% decrease) and old (57%) cells as compared to the control. Similarly, HC treatments were more effective in reducing lipofuscin accumulation in young cells for 4weeks (31% decrease) and old cells (63%). The present results show that the free-radical scavenging in the cultured fibroblasts reduced the lipofuscin accumulation.

• 社団法人日本動物学会の論文
• 2001-01-20

著者

• Okada Yoshinori

  Laboratory On Ageing Chukyo Junior College

• OKADA Mizue

  Department of Hygiene, Gifu University School of Medicine

• Okada Mizue

  Department Of Hygiene Gifu University School Of Medicine

• Okada Yoshinori

  Laboratory On Ageing And Health Management Aichi Prefectural College Of Nursing And Health

関連論文

• Increase of the Cellular Growth of Old Human Diploid Fibroblasts by Radical Scavenger : Methanolic Extract of Broad Beans
• Scavenging Effects of Methanolic Extracts of Broad Beans on Free-Radical Species
• Effects of the Radical Scavenger, Water Soluble Protein from Broad Beans on Lipofuscin, Cathepsin B, Cell Growth in Human Lung Fibroblasts(Cell Biology)
• Effect of a Radical Scavenger "Water Soluble Protein" from Broad Beans (Vicia faba) on Antioxidative Enzyme Activity in Cellular Aging
• INHIBITORY EFFECT OF NONYLPHENOL BINDING BY METHANOLIC EXTRACTS FROM EDIBLE PLANTS ON THE RECEPTOR OF CULTURED RAT HEPATOCYTES(Behavior Biology and Ecology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• Effects of Methanolic Extracts from Broad Beans on Cellular Growth and Antioxidant Enzyme Activity
• Effects of Radical Scavenger Protein from Broad Beans on Glutathione Status in Human Lung Fibroblasts
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