Effects of Radical Scavenger Protein from Broad Beans on Glutathione Status in Human Lung Fibroblasts
スポンサーリンク
概要
- 論文の詳細を見る
Objective: Human diploid cells are more susceptible to oxidative stress at late passage than at early passage, presumably because of the decrease in cellular-reduced glutathione (GSH) concentration. Water-soluble protein (WSP) from broad beans scavenges free radicals. The effects of WSP on the glutathione system were examined in PDL 20 (early passage) and PDL 50 (late passage) human lung fibroblasts (TIG-1). Methods: To determine cytosolic glutathione peroxidase (GSH-Px) activities, glutathione reductase (GR) activities, oxidized glutathione (GSSG) concentrations, and GSSG/reduced glutathione (GSH) ratios, WSP and hydrocortisone (HC) treatments of TIG-1 cells (PDL 20→50 and PDL 50→75) were performed for 40 days. We also investigated the GSSG concentrations and GR activities in PDL 20 cells that were continuously treated with WSP until PDL 39 and 55. Results: GSSG concentrations decreased in WSP- and HC-treated PDL 50→75 cells. The GSSG/GSH ratios in PDL 50→75 cells became low after the treatments. Increases in GR activities were observed in treated PDL 50→75 cells. The decline in the GSSG concentration of PDL 50→75 cells correlated with the increase in GR activity. The GSSG levels in control cells were higher following cellular age, whereas the levels in treated cells were lower than those in the control. The studies on cellular age-related changes indicated that greater increases in GR activity were found in treated cells than in the control. Conclusion: These results indicated that WSP influences the GSSG concentration that is associated with cellular aging, but the mechanism of GSSG reduction by WSP remains unknown. The enhancement of glutathione status following WSP treatment may be related to the delay in the cellular aging.
- 日本衛生学会の論文
- 2007-11-01
著者
-
Okada Yoshinori
Laboratory On Ageing Chukyo Junior College
-
Okada Mizue
Nutrition Section Yms Laboratory
-
Okada Yoshinori
Laboratory On Ageing And Health Management Aichi Prefectural College Of Nursing And Health
-
Okada Mizue
Nutrition Section Yms Lab.
関連論文
- Increase of the Cellular Growth of Old Human Diploid Fibroblasts by Radical Scavenger : Methanolic Extract of Broad Beans
- Scavenging Effects of Methanolic Extracts of Broad Beans on Free-Radical Species
- Effects of the Radical Scavenger, Water Soluble Protein from Broad Beans on Lipofuscin, Cathepsin B, Cell Growth in Human Lung Fibroblasts(Cell Biology)
- Effect of a Radical Scavenger "Water Soluble Protein" from Broad Beans (Vicia faba) on Antioxidative Enzyme Activity in Cellular Aging
- INHIBITORY EFFECT OF NONYLPHENOL BINDING BY METHANOLIC EXTRACTS FROM EDIBLE PLANTS ON THE RECEPTOR OF CULTURED RAT HEPATOCYTES(Behavior Biology and Ecology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Effects of Methanolic Extracts from Broad Beans on Cellular Growth and Antioxidant Enzyme Activity
- Effects of Radical Scavenger Protein from Broad Beans on Glutathione Status in Human Lung Fibroblasts
- Lifestyle and Colorectal Cancer : A Case-control Study
- Is Interest in Art Effective in Health-Related Quality of Life? : Results of a Cross-Sectional Survey on Lifestyle and Health Promotion
- Application of telepsychiatry : A preliminary study