Effects of Methanolic Extracts from Broad Beans on Cellular Growth and Antioxidant Enzyme Activity

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概要

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• Objective: There are several reports of cellular-aging-dependent alterations in the antioxidant capacity of human fibroblasts. Fibroblasts show slower the growth rate at late passages (referred to hereafter as old cells) than at early passages (referred to hereafter as young cells). Antioxidants may control cellular growth by modulating reactive oxygen species (ROS). Methanolic extracts from broad beans (MEBB) contain phenolic compounds and have ROS-scavenging activities. In this study, we investigated the effects of MEBB on cellular growth and antioxidant levels in normal human lung fibroblasts. Methods: To determine cytosolic superoxide dismutase (SOD) activities, cytosolic glutathione peroxidase (GSH-Px) activities, catalase activities, reduced glutathione (GSH) concentrations, and growth rate, MEBB treatments were performed on young and old cells. Results: In young and old cells treated with 120 μg/ml MEBB, the growth rates increased by 28.1 and 15.2%, respectively, compared with controls. The MEBB treatment of young cells caused a 62.5% increase in SOD activity, but the treatment of old cells caused a 39.5% decrease. The catalase activities of the young and old cells treated with MEBB were equal to those of control cells. Young and old cells treated with MEBB were equal to the control cells in terms of GSH-Px activity. The GSH concentrations in the young and old cells treated with 120 μg/ml MEBB increased by 22.1 and 45.9%, respectively. Conclusion: These studies elucidated a new cellular growth mechanism whereby human lung fibroblasts modulate intracellular GSH levels via the action of MEBB.

• 日本衛生学会の論文
• 2007-11-01

著者

• Okada Yoshinori

  Laboratory On Ageing Chukyo Junior College

• Okada Mizue

  Nutrition Section Yms Laboratory

• Okada Yoshinori

  Laboratory On Ageing And Health Management Aichi Prefectural College Of Nursing And Health

• Okada Mizue

  Nutrition Section Yms Lab.

関連論文

• Increase of the Cellular Growth of Old Human Diploid Fibroblasts by Radical Scavenger : Methanolic Extract of Broad Beans
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• Effect of a Radical Scavenger "Water Soluble Protein" from Broad Beans (Vicia faba) on Antioxidative Enzyme Activity in Cellular Aging
• INHIBITORY EFFECT OF NONYLPHENOL BINDING BY METHANOLIC EXTRACTS FROM EDIBLE PLANTS ON THE RECEPTOR OF CULTURED RAT HEPATOCYTES(Behavior Biology and Ecology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
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