Tetrahymena Elongation Factor-1α Binds to Hsp70 Family Proteins

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概要

• 論文の詳細を見る

• Translation elongation factor 1α (EF-1α) catalyzes the GTP-dependent binding of aminoacyl-tRNA to the ribosome. We previously reported that Tetrahymena EF-1α induced bundles of rabbit skeletalmuscle F-actin as well as Tetrahymena F-actin (Kurasawa et al., (1996) Zool. Sci. 13: 371–375), andthat Ca2+/calmodulin (CaM) regulated the F-actin-bundling activity of EF-1α without inhibition of the bindingbetween EF-1α and F-actin (Kurasawa et al.,(1996) J. Biochem. 119: 791–798). In this study, we investigatedEF-1α-binding proteins in Tetrahymena using a Tetrahymena EF-1α affinity column. TetrahymenaEF-1α bound directly to 74 kDa, 77 kDa, and 78 kDa proteins, in addition to CaM. The bindings of 74 kDa, 77kDa, and 78 kDa proteins to Tetrahymena EF-1α were Ca2+-independent and ATP-sensitive. The N-terminalamino acid sequence of the 74 kDa protein was similar to those of 70 kDa heat shock protein (hsp70) family.

• 社団法人日本動物学会の論文
• 1999-04-15

著者

• Numata Osamu

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Numata Osamu

  Institute of Biological Sciences University of Tsukuba

• Numata O

  Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Numata Osamu

  Institute Of Biological Sciences Tsukuba University

• Watanabe Y

  Jobu University

• KURASAWA Yasuhiro

  Institute of Biological Sciences,University of Tsukuba

• WATANABE Yoshio

  Jobu University

• Watanabe Y

  Tokyo Inst. Technol. Yokohama

• Kurasawa Yasuhiro

  Institute Of Biological Sciences University Of Tsukuba

関連論文

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• IDENTIFICATION OF NOVEL ACTIN-RELATED PROTEINS AND A SMALL G PROTEIN IN TETRAHYMENA THERMOPHILA(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
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• ANALYSIS OF TCBP-25 (TETRAHYMENA CA^-BINDING PROTEIN OF 25 kDa) DURING SEXUAL REPRODUCTION IN TETRAHYMENA THERMOPHILA(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• Immunofluorescence Localization of a 23-kDa Tetrahymena Calcium-Binding Protein, TCBP-23, in the Cell Cortex
• Localization of Microtubules during Macronuclear Division in Tetrahymena and Possible Involvement of Macronuclear Microtubules in 'Amitotic' Chromatin Distribution
• Tetrahymena Nuclear Proteins that Bind to a Micronucleus-Specific Sequence during Vegetative Growth
• Enzymatic Form and Cytoskeletal Form of Bifunctional Tetrahymena 49kDa Protein Is Regulated by Phosphorylation(Cell Biology)
• Tetrahymena Fimbrin Localized in the Division Furrow Bundles Actin Filaments in a Calcium-Independent Manner
• PRODUCTION OF RECOMBINANT TETRAHYMENA FIMBRIN AND ITS CHARACTERIZATION(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• The Dual Functions of Tetrahymena Citrate Synthase Are Due to the Polymorphism of Its Isoforms
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