Characterization of F-Actin Bundling Activity of Tetrahymena Elongation Factor 1α Investigated with Rabbit Skeletal Muscle Actin

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概要

• 論文の詳細を見る

• Elongation factor 1α (EF-1α) is an essential factor for protein synthesis in eukaryotes. Here, we demonstrated that Tetrahymena EF-1α induced bundles of rabbit skeletal muscle F-actin as well as Tetrahymena F-actin in vitro, although Tetrahymena and skeletal muscle actins are different in some parts of their primary structures and in the binding abilities to some actin-binding proteins. Co-sedimentation experiments showed that the binding ratio of Tetrahymena EF-1α to skeletal muscle F-actin in the bundles was 1 : 1. Electron microscopic observation showed that alkaline pH or high ionic strength reduced the bundling activity of Tetrahymena EF-1α to some extent, although the EF-1α seemed to be able to induce bundling of the F-actin within the range of physiological condition.

• 社団法人日本動物学会の論文
• 1996-06-15

著者

• Numata Osamu

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Numata Osamu

  Institute of Biological Sciences University of Tsukuba

• Numata O

  Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Numata Osamu

  Institute Of Biological Sciences Tsukuba University

• Watanabe Y

  Jobu University

• WATANABE Yoshio

  Joubu University

• KURASAWA Yasuhiro

  Institute of Biological Sciences,University of Tsukuba

• Watanabe Y

  Tokyo Inst. Technol. Yokohama

• Kurasawa Yasuhiro

  Institute Of Biological Sciences University Of Tsukuba

関連論文

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• DISTRIBUTION OF STEROL-RICH MEMBRANE DOMAINS IN TETRAHYMENA DURING CYTOKINESIS(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• IDENTIFICATION OF NOVEL ACTIN-RELATED PROTEINS AND A SMALL G PROTEIN IN TETRAHYMENA THERMOPHILA(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• OOLONG TEA ACTIVATES THE MITOCHONDRIAL MEMBRANE POTENTIAL AND SWIMMING VELOCITY IN TETRAHYMENA AND MOUSE SPERM(Cell Biology and Morphology & Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Jap
• Determination of Division Plane and Organization of Contractile Ring in Tetrahymena
• Calmodulin and Ca^/Calmodulin-Binding Proteins are Involved in Tetrahymena thermophila Phagocytosis
• Determination of Division Plane and Organization of Contractile Ring in Tetrahymena
• A NOVEL CLASS XIX MYOSINS FROM TETRAHYMENA THERMOPHILA(Cell Biology and Morphology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• Citrate Synthase Purified from Tetrahymena Mitochondria Is Identical with Tetrahymena 14-nm Filament Protein^1
• High-Molecular-Weight Polyphenols from Oolong Tea and Black Tea: Purification, Some Properties, and Role in Increasing Mitochondrial Membrane Potential
• Tetrahymena Eukaryotic Translation Elongation Factor 1A (eEF1A) Bundles Filamentous Actin through Dimer Formation
• TETRAHYMENA ELONGATION FACTOR-1α (EF-1α) BUNDLDES F-ACTIN BY FOEMING A DIMER(Cell Biology and Morphology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• TETRAHYMENA ELONGATION FACTOR-1α (EF-1α) BUNDLES FILAMENTOUS ACTIN BY FORMING A DIMER : A NOVEL EVIDENCE OF THE EF-1α DIMER MODEL(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• The Mutant Gene Product of a Tetrahymena Cell-Division-Arrest Mutant cdaA Is Localized in the Accessory Structure of Specialized Basal Body Close to the Division Furrow
• High-Molecular-Weight Polyphenols from Oolong Tea and Black Tea : Purification, Some Properties, and Role in Increasing Mitochondrial Membrane Potential
• Tetrahymena Elongation Factor-1α Is Localized with Calmodulin in the Division Furrow^1
• Tetrahymena Elongation Factor-1α Binds to Hsp70 Family Proteins
• A New Tetrahymena Actin-Binding Protein Is Localized in the Division Furrow^1
• Characterization of F-Actin Bundling Activity of Tetrahymena Elongation Factor 1α Investigated with Rabbit Skeletal Muscle Actin
• F-Actin Bundling Activity of Tetrahymena Elongation Factor 1α Is Regulated by Ca^/Calmodulin^1
• Polymerization of Highly Purified Tetrahymena 14-nm Filament Protein/Citrate Synthase into Filaments and Its Possible Role in Regulation of Enzymatic Activity
• IDENTIFICATION AND MOLECULAR CLONING OF RADIAL SPOKE PROTEIN 4 AND 6 AS CA2+/CALMODULIN-ASSOCIATING PROTEINS IN TETRAHYMENA CILIA(Cell Biology and Morphology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• FUNCTIONAL ANALYSIS OF TETRAHYMENA Ca^-BINDING PROTEIN OF 25 KDA (TCBP-25) USING ANTISENSE RIBOSOMAL RNA METHOD(Cell Biology and Morphology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• ANALYSIS OF TCBP-25 (TETRAHYMENA CA^-BINDING PROTEIN OF 25 kDa) DURING SEXUAL REPRODUCTION IN TETRAHYMENA THERMOPHILA(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• Immunofluorescence Localization of a 23-kDa Tetrahymena Calcium-Binding Protein, TCBP-23, in the Cell Cortex
• Localization of Microtubules during Macronuclear Division in Tetrahymena and Possible Involvement of Macronuclear Microtubules in 'Amitotic' Chromatin Distribution
• Tetrahymena Nuclear Proteins that Bind to a Micronucleus-Specific Sequence during Vegetative Growth
• Enzymatic Form and Cytoskeletal Form of Bifunctional Tetrahymena 49kDa Protein Is Regulated by Phosphorylation(Cell Biology)
• Tetrahymena Fimbrin Localized in the Division Furrow Bundles Actin Filaments in a Calcium-Independent Manner
• PRODUCTION OF RECOMBINANT TETRAHYMENA FIMBRIN AND ITS CHARACTERIZATION(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• The Dual Functions of Tetrahymena Citrate Synthase Are Due to the Polymorphism of Its Isoforms
• F-Actin Bundling Activity of Tetrahymena Elongation Factor 1.ALPHA. Is Regulated by Ca2+/Calmodulin.

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