Tetrahymena Nuclear Proteins that Bind to a Micronucleus-Specific Sequence during Vegetative Growth
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概要
- 論文の詳細を見る
Tetrahymena thermophila has two nuclei: a micronucleus is transcriptionally silent during vegetative growth and a macronucleus is active. Extensive programmed DNA rearrangement is known to occur during the development of the somatic macronucleus from the germ-line micronucleus. We previously found a 1.4 kb micronucleus-specific sequence, C-element, which was located upstream of the micronuclear calmodulin gene and was eliminated from the macronuclear genome during macronuclear development. Here, using gel mobility shift assays, we show that C-element binding factors, CBFs, are present in the nuclear extract prepared from vegetative cells. Competition experiments demonstrate that CBFs bind to two regions within the C-element. A sequence motif common to these regions is 5'-ATAGATTT-3'.
- 社団法人日本動物学会の論文
- 1996-08-15
著者
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Numata Osamu
Institute Of Biological Sciences Tsukuba University
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WATANABE Yoshio
Joubu University
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Katoh M
Aichi Human Service Center Aichi Jpn
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Katoh Mariko
Institute Of Biological Sciences University Of Tsukuba
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