Necessity of Oviducal Pars Recta Secretions for the Formation of the Fertilization Layer in Xenopus laevis(Developmental Biology)

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In un fertilized uterine eggs of Xenopus laevis, there is a layer between the vitelline coat (VC) and the innermost jelly layer (J_1) which shows the same distribution pattern as the fertilization (F) layer in a fertilized egg. This layer, referred to as a pre-fertilization (PF) layer, was present in eggs from the posterior one-fifth of the pars recta of the oviduct (pars recta 2), where no jelly is secreted. An F layer formed after activation of eggs possessing the PF layer was resistant to mercaptan treatment, whereas a layer produced in activated eggs having no PF layer was dissolved by the same treatment. Immunofluorescent observations demonstrated that an antigen(s) specifically localized in the cells of the pars recta 2 is deposited in the VC of eggs passing through this short oviducal portion. In immunodifTusion analyses, this antigen was found in the EDTA-extracted F-material and the VC of activated uterine eggs, but not in the VC of coelomic eggs, J_1, or cortical granule exudates. It is postulated that the F layer in Xenopus is formed by association of cortical granule exudates with the preexisting PF layer distinct from J_1.
社団法人日本動物学会の論文