Sodium Accumulation in Decomposing Yolk Platelets during the Development of Xenopus laevis(Developmental Biology)
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概要
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To cytochemically demonstrate the accumulation of sodium ions in decomposing yolk platelets, we first aimed to develop a method of sodium detection by administrating the magnesium uranyl acetate (double acetate) reagent, which throws down the sodium as triple acetate, to sciatic nerves of Xenopus laevis. By observing the cell contour and the volume of the precipitates produced in the intercellular and interlamellar spaces of the myelin sheath around the nerves, we determined that a 15-times dilution of the original double acetate reagent (Caley and Foulk,1929) was the best concentration for biological usage. The validity of the double acetate method was assessed by observing the specific localization of triple acetate precipitates in the skeletal muscle and kidney ; In addition to their localization in intercellular spaces, the precipitates were preferentially present in the transverse system of skeletal muscle cells and present in the ground cytoplasm as well as in the organelles other than vacuoles of proximal convoluted tubule cells of the kidney. By applying this method to developing embryos, it was found that the sodium ions are stored in the vesicles during the cleavage stages of development, are apparently transported by the vesicles to decomposing yolk platelets at the early neural plate and later stages, and are accumulated in these platelets. Those results would satisfy the prediction that the sodium concentration must be increased in decomposing yolk platelets, since yolk solubilization by high salt concentrations is prerequisite for amphibian yolk digestion to occur.
- 社団法人日本動物学会の論文
- 1999-02-15
著者
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YOSHIZAKI Norio
Department of Animal Resource Production, United Graduate School of Agricultural Science, Gifu Unive
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Yoshizaki Norio
Department Of Biological Diversity Faculty Of Agriculture Gifu University
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Yoshizaki Norio
Department Of Agricultural Science Faculty Of Applied Biological Science Gifu University
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Yoshizaki Noriko
Department of Biological Diversity, Faculty of Agriculture, Gifu University
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