哺乳類赤血球の細胞質放出現象と血小板及各種白血球形成との關係 : II.血液培養及塗抹標本に於ける中性嗜好性白血球の形成領域其他

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The materials and methods used in these studies were the same as that of my previous report. In this paper the results of my studies on the formation of neutrophilic leucocytes, "field of leucocyte-formation" and some of other experimental results in blood cell cultures and blood-smear preparation are presented. The phenomenon of cytoplasm-extrusion of erythrocyte in mammal takes place in somewhat slighter degree than that of bird, reptile or amphibian, and this phenomenon has important effect upon the blood coagulation so that it correlates to the difference of the rapidity of blood coagulation between mammal and bird. In the blood-cell culture or on the thick blood layer of the smear preparation there can be seen several side of "leucocyte-formation areas, " which are made up of following elements: (i) lymphocytes and monocytes transformed from extruded cytoplasm, (ii) extruded cytoplasm itself, (iii) transitional forms (proleucocytes) between extruded cytoplasm and leucocytes, (iv) remnants of erythrocytes or transitional forms from these remmants into leucocytes. These areas consist of the fused mass of above mentioned elements varying in number from 5 or 6 to several hundreds of them. Of the same blood of an animal, the numerical ratio of leucocytes to erythrocytes on the blood-smear preparation can be altered by means of giving the mechanical actions such as fraction or pressure against the erythrocytes at the time of smearing and by changing the thickness of blood layer on the preparation. From these facts it can not be assertained that the blood-figures of the blood smear preparation always indicate the same as that of circulating blood in living animals. It can be shown that (i) in blood cell-culture, the erythrocytes become fewer and leucocytes become more numerous after 24 to 48 hours, while, (ii) there can hardly be seen mitotic figures of either erythrocytes or of leucocytes. Moreover, spherical erythrocytes themselves show a sign of transitional staining character into small lymphocytes, (iii) the extruding condition of cytoplasm out of erythrocytes can be actually recognized by continuous watching of living erythrcytes, (iv) on blood-smear preparation, there are many of transitional forms from the extruded cytoplasm into the leucocytes or blood platelets and there are many of connected conditions between these elemsnts (leucocytes, blood plateletes or extruded cytoplasm) and erythrocytes. It was discussed that so-called the phenomena of "pinocytosis" defined by Ropes and of the "erythrocytes engorged by macro-phage with phagocytosis" probably correspond to a certain stage of "cytoplasm-extrusion of erythrocytes" accompanied by the differentation of erythrocytes. The phenomenon of the "cytoplasm-extrusion of erythrocytes" has close connections with the following factors, (i) change of enviromental factors of blood cells and stop of blood curent by pouring out the blood, (ii) adhering of erythrocytes to the foreign body, (iii) mechanical action against erythrocytes, (iv) thickness of blood layer smeared on the slide glass. From these points of view, the erythrocyte might be looked upon as a very primitive and an undifferentiated cell, of which nuclear substance has not yet made its appearance.
社団法人日本動物学会の論文
1950-01-15

哺乳類赤血球の細胞質放出現象と血小板及各種白血球形成との關係 : II.血液培養及塗抹標本に於ける中性嗜好性白血球の形成領域其他

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