バフンウニの卵の細胞質構造に關する1新知見

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The results of a further investigation on the cytoplasmic structure of the egg of a sea-urchin, Strongylocentrotus Pulcherrimus, is reported in this paper. The author found a sort of granules scattered in the cortical cytoplasm of the unfertilized egg, and named them "janus green granules". The granules stain intensely with a solution of Janus green B n sea water in living state. For the fixation of the granules, CHAMPY'S fluid was used. The paraffin sections of the fixed materials were brought through xylol, absolute, and 95 per cent alcohol into 0.5 N. NaOH aqueous solution. After twenty minutes the sections were washed with water, and then stained on a slide for one minute over a flame, with one per cent aqueous solution of Janus green B. After cooling, the sections were differentiated with water and alcohol, and then embedded n Canada balsam through alcohol and xylol. The Janus green granules stained dark blue, the yolk granules light grey and the nucleus remained colorless. The formalin material was also stainable with a dilute aqueous solution of janus green B, when the material was previously treated with 0.5 N. NaOH solution. The Janus green granules have similarities to mitochondoria in the points that they are stained with Janus green B in fresh material, and that they are unstable to acids. They are distinguishable from mitochondria in not being stained with acid fuchsin. The distribution of the Janus green granules in the mature egg was confined to the peripheral portion of the egg cytoplasm. They were dispersed in the cytoplasm of the egg of the stage of the 1st oocyte. The author is inclined to believe that the migration of the janus green granules to the peripheral portion of the egg cytoplasm will occur within the period between the maturation division and the oviposition.
社団法人日本動物学会の論文
1936-10-15

バフンウニの卵の細胞質構造に關する1新知見

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