鳥類の精巣発育と視床下部・下垂体神経分泌系I. : 顕微測光による正中隆起部神経分泌物の定量化

概要

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A method for quantitative measurement of the neurosecretory materials has been devised in order to investigate the relationships between changes of the neurosecretory materials in the avian median eminence (ME) and photoperiodic testicular development. This method consists of microphotometry of serial sections of ME stained by aldehyde-thionin (AT). If the optical density of a section i is-log Ti, the area is Bi, and the number of sections stainable with AT is l, the total amounts of materials are calculated from the equation: M=Σ^^l__<i=1>Bi(logTi^<-1>) Besides the value M, average Ootical Density, average Area and average Optical Density×Area were calculated and average Optical Density of the neurohypophysis was also obtained. The mutual relations among these values were examined. In Japanese quails tested here, staainable numbers of serial sections were 64-98, av. OD was 0.04-0.16, av. Area was 1.6-3.5, av. OD×Area was 0.12-0.34, and M was 9-25. The results indicate that neurosecretory materials in ME are richer in birds under long daily photoperiods than in birds subjected to short days.
社団法人日本動物学会の論文
1965-10-15