ドジョウMisgurnus anguillicaudatus(CANTOR)腸管の酸性及びアルカリ性フォスファクターゼの組織化学的分布について

概要

論文の詳細を見る
According to Minobe (1924), the alimentary tract of loach is simple in shape and almost straight, and the tract has two different portions. The anterior 1/3 of the tract is the digestive-absorptive portion with a cylindrical epithelium while the posterior part is the respiratory portion with a cubicflat epithelium. The transitional part is twisted. The present author attempted to detect acid and alkaline phosphatases in the alimentary tract of the loach to prove the different functions of both portions, employing Gomori's and Yamao's histochemical methods. Both groups of loaches starved and fed for several months were used for this study. The substrates were Na-β-glycerophosphate, RNA, DNA and FMN. The reaction of alkaline phosphatase (Fig. 1-8) wes generally strong as compared with acid phosphatase (Figs. 9-12). Alkaline phosphatase was distributed almost in the external layer of the epithelium and acid phosphatase was found throughout the epithelium. In the reaction of the fed loach to Na-β-glycerophosphate, alkaline phosphatase was distributed markedly in the epithelium of both digestive-absorptive (Fig. 8) and respiratory portions. In the starved loach, the same enzyme was found in less amount in the epithelium of the whole alimentary tract (Fig. 5, digestive-absorptive portion; 6, respiratory portion). The reaction of alkaline phosphatase to RNA was almost similar to the reaction to Na-β-glycerophosphate (Fig. 4, digestive-absorptive portion of fed loach), except for that the distribution in the respiratory portion of the starved loach was not detected. The FMN alkaline phosphatase was not detected in the respiratory portion of the fed loach, but it was remarkable in the epithelium in the digestive-absorptive portion (Fig. 3). In the starved loach, weak reaction of FMN alkaline phosphatase was observed in the digestive-absorptive portion and no reaction in the respiratory portion. The DNA alkaline phosphatase was strongly positive in the digestive-absorptive portion (Fig. 1, fed) but there was almost no activity in the respiratory portion of unstarved loach. It was nearly the same in the starved loach (Fig. 2, digestive-absorptive portion). The reaction of acid phosphatase was postive only to Na-β-glycerophosphate. The distribution was observed in the whole epithelium of the alimentary tract of the fed loach (Fig. 9 and 10, digestive-absorptive portion; 11, transitional part; 12, respiratory portion). Acid phosphatase reaction was negative when RNA, DNA and FMN were used as substrate both in fed and starved loaches. These observations suggest that the two parts of the alimentary tract of the loach, the digestive-absorptive and the respiratory portions, are different in function.
社団法人日本動物学会の論文
1963-04-15

ドジョウMisgurnus anguillicaudatus(CANTOR)腸管の酸性及びアルカリ性フォスファクターゼの組織化学的分布について

スポンサーリンク

概要

著者

関連論文

スポンサーリンク