サンショウウオ後肢直前位置への肢移植により生じた過剰肢材料の由来に関するオートラジオグラフィーによる研究

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Two kinds of supernumerary limbs were obtained following the transplantation of limb-bud at the site just anterior to the hind-limb in larvae of Hynobius. The one kind (ST-limb) was intimately associated with the transplanted fore-limb, and the other kind (SN-limb) was closely linked with the normal hind-limb (USUI, 1967). The present experiments were performed with the purpose of knowing the material contribution of the transplant to these supernumerary limbs by transplanting ^3H-labeled limb-bud. The results showed that the labeled material was seen in most of the supernumerary limbs, ST- as well as SN-limbs, as if the material from transplants contribiuted to the formation of supernumerary limbs of both kinds (Table 1). However, the supernumerary limbs induced by the transplantation of labeled Bufo limb-bud in Hynobius were found to be also labeled, notwithstanding that they were clearly formed of the host cells (Table 2). It can not be concluded, therefore, that all the labeled supernumerary limbs are formed with a contribution of the material originated from the transplants. From one to three days after the transplantation, many labeled granules, which seemed to be originated from the decomposed labeled nuclei of the transplant cells, were found on the border between the host tissue and the transplant. This fact suggested the possibility that the labeled DNA precursor released by the lysis of cells of the transplant might be reutilized by the host cells forming supernumerary limbs. Autoradiographic method did not seem to be suitable for the study of such problem as the material origin of the supernumerary limbs, the formation of which is not recognizable until many days after the transplantion.
社団法人日本動物学会の論文
1970-03-15

サンショウウオ後肢直前位置への肢移植により生じた過剰肢材料の由来に関するオートラジオグラフィーによる研究

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