電気生理学的にみた両生類卵の卵割面膜形成
スポンサーリンク
概要
- 論文の詳細を見る
1. Changes of effective resistance and membrane potential of the newt (Triturus pyrrhogaster) eggs and their macroblastomeres during cleavage were studied by microglass electrode technique in order to obtain information of membrane formation at the cleavage furrow. 2. Effective resistances of intact eggs averaged 2M ohm at the onset of the primary furrow formation, decreased with extension of the furrow and attained to the minimum value (520K ohm) at the secondary furrow formation. Subsequently, the resistances increased again up to 800K ohm at the end of the first cleavage. The same trend of changes in the resistances was observed at the second cleavage. Electrical coupling (ratio v^2/v^1 of electrotonic potentials recorded from two blastomeres) was constant of 1 during the 1st cleavage, and of more than 0.8 at the 2nd cleavage. Negative change in the membrane potential occurred concomitantly with the change in the resistance. Sometimes, no recovery of the electrical change was observed during the course of the 2nd cleavage. This seems to be correlated with the absence of pigmented surface layer at the cleavage furrow of the animal pole. 3. No change in membrane potential and a decrease in effective resistance of isolated blastomeres were observed during cleavage. The resistance decreased at the start of cleavage, and attained to the minimum value when the blastomeres appeared to be separated almost into two daughter cells. Subsequently, the resistance at the end of cleavage raised to 3-5 times of the minimum value. Electrical coupling between the daughter cells decreased below the ratio of 1 at the stage of minimum value of resistance, attaining the ratios of 0.3 to 0.8 at the end of cleavage. 4. From these results, it may be supposed that new membrane, which is more permeable to ions than pre-existing plasma membrane of the blastomeres, is produced at the cleavage furrow and exposed electrically to the external medium during cytokinesis. The furrow is hermetically sealed by the surface layer of the pre-existing membrane (perijunctional insulation). Electrical changes of the intact egg during cleavage may be interpretable by two factors, new membrane formation and enormous resistive barrier located on the surface of the egg.
- 社団法人日本動物学会の論文
- 1968-10-15
著者
関連論文
- イモリ胚心筋細胞の培養下の増殖(発生学)
- イモリ胚表皮細胞の活動電位の温度依存性(生理学)
- イモリ胚表皮細胞よりの活動電位(発生学)
- イモリ胚の細胞間電気的連絡の低pHによる阻害(発生学)
- イモリ遊離割球細胞間のイオン流通・阻害剤の効果(発生学)
- イモリ遊離割球細胞間のイオン流通の形成(発生)
- 成熟, 付活過程におけるカエル卵の電気的特性(発生学)
- 移植によるイモリ胚表皮活動電位の早期出現(発生学)
- イモリ予定表皮細胞の興奮性発現の可塑性(発生学)
- イモリ初期胚の胚葉間の電気的連絡(発生学)
- 日本動物学会第49回大会大会記事
- ニュクリポアーメンブランフィルターを介しての割球細胞間のイオン流通(発生学)
- イモリ遊離割球細胞間のイオン流通・阻害剤の効果(発生学)
- イモリ遊離割球の接触による接合部膜形成(発生)
- 両生類卵の付活電位(発生)
- 48 ウニ卵の付活電位とイオンの影響
- 電気生理学的にみた両生類卵の卵割面膜形成
- Fundulus(メダカ科)卵の付活と電気的性質
- イモリ卵の膜電粒(発生・実験形態)
- 海棲メダカの研究 : III.受精並びに発生に対する塩分濃度の影響
- 海棲メダカの研究 : II.淡水メダカの海水適応
- 海棲メダカの研究 : I.野外観察