A Protein Kinase Inhibitor H-7 Induces Process Extrusion in Fetal Rat Thyroid C-Cells in vitro
スポンサーリンク
概要
- 論文の詳細を見る
Calcitonin-producing cells (C-cells) are endocrine cells derived from the neural crest. We examined the effects of three types of protein kinase inhibitors on the induction of neuronal phenotypes in the rat thyroid C-cells in vitro. In a primary culture of 16-day-old fetal rat thyroid glands, 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride (H-7, 25-75 μM) induced both process extrusion and expression of highly polysialylated neural cell adhesion molecule (NCAM) in the C-cells. These effects of H-7 were completely prevented by okadaic acid, a potent protein phosphatase inhibitor. In contrast to H-7, selective inhibitors for cyclic nucleotide-dependent protein kinases such as N-(2-guanidinoethyl)-5-isoquinolinesulfonamide hydro-chloride (HA1004, 25-200 μM) and N-[2-(p-bromocinnamylamino) ethyl]-5-isoquinolinesulfonamide (H-89, 0.25-20 μM) failed to induce process extrusion or the expression of highly polysialylated NCAM in fetal rat C-cells. In cultured C-cells of adult origin, H-7 failed to induce marked process elongation or the expression of highly polysialylated NCAM. These results suggest that the morphological plasticity of the fetal C-cells depends upon the degree of phosphorylation of some proteins, and that the plasticity of adult C-cells are more restricted than that of fetal origin.
- 社団法人日本動物学会の論文
著者
-
Seki Tatsunori
Department Of Anatomy Juntendo University Of Medicine
-
Nishiyama Ichiro
Komazawa Women's Junior College
-
Ogiso Manabu
Cell and Information, PRESTO, Japan Science and Technology Corporation (JST)
-
Oota Tadachika
Isotope Center, Tokyo University of Agriculture
-
Ogiso Manabu
Cell And Information Presto Japan Science And Technology Corporation (jst)
-
Oota T
Hokkaido Univ. Education Iwamizawa Jpn
-
Nishiyama Ichiro
Komazawa Women's Junior College
関連論文
- Seizures Decrease Postnatal Neurogenesis and Granule Cell Development in the Human Fascia Dentata
- The isolation of neural stem cells from the olfactory bulb of Parkinson's disease model
- 成体のニューロン新生におけるポリシアル酸発現細胞
- Effect of Hypothalamic Extract on the Prolactin Release from the Bullfrog Pituitary Gland with Special Reference to Thyrotropin-Releasing Hormone (TRH) : Endocrinology
- Involvement of highly polysialylated neural cell adhesion molecule (PSA-NCAM)-positive granule cells in the amygdaloid-kindling-induced sprouting of a hippocampal mossy fiber trajectory
- Straying phenomenon of migrating LHRH neurons and highly polysialylated NCAM in the chick embryo
- Effects of Ergocornine and Reserpine on Metamorphosis in Bufo bufo japonicus Tadpoles
- Developmental anomalies of the enteric nervous system in normoganglionic segments of bowel from rats with total colonic aganglionosis
- Supportive Role of Cellular Bridge of Neurons Expressing a Highly Polysialylated Form of NCAM (NCAM-H) at the Initial Stage of Migration of LHRH Neurons
- ROLE OF THE OLFACTORY PLACODE DERIVED MIGRATORY CELLS IN DEVELOPMENT OF THE OLFACTORY NERVE(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- NEUROGENESIS AND REGENERATION IN THE MAMMALIAN BRAIN
- A Protein Kinase Inhibitor H-7 Induces Process Extrusion in Fetal Rat Thyroid C-Cells in vitro
- Morphology of the Skin Glands of the Crab-eating Frog : Rana cancrivora
- Microenvironmental elements supporting adult hippocampal neurogenesis
- Neuron-like morphology expressed by perinatal rat C-cells in vitro
- Responses of prolactin cells in the adenohypophysis of bullfrog tadpoles to dopamine agonist and antagonist.