Isolation and Some Characterization of Vitellogenin and Its Related Egg Yolk Proteins from Coho Salmon : Oncorhynchus kisutch
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概要
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Vitellogenin (Vg) and its related egg protein 1 (E1) and egg protein 2 (E2) were isolated from serum or eggs of mature female coho salmon by precipitation in distilled water followed by chromatography on Sepharose 6B (Vg) or Sephadex G-200 (yolk proteins). The coho salmon proteins reacted specifically with respective antisera raised against Vg (a-Vg), E1 (a-E1) or E2 (a-E2) purified from chum salmon. Two female-specific proteins were identified in serum from mature coho salmon. Coho salmon Vg had an aparent molecular weight of 540 kDa after chromatography on Superose 6, appeared as a major 240 kDa band and a minor 165 kDa band in SDS-PAGE, and resolved into a major 165 kDa band and several minor 70-150 kDa bands after SDS-PAGE under reducing conditions. It reacted immunologically with a-E1 and a-E2. The other female-specific serum protein, designated coho salmon E2, reacted with a-E2 but not with a-E1. The apparent molecular weight of purified E1 and E2 were 230 and 35 kDa, respectively, after chromatography on Superose 6. E1 appeared as two main bands of 150 and 92 kDa in SDS-PAGE which resolved into two smaller bands (92 kDa and 20 kDa)after reduction. E2 appeared as a 30 kDa band in SDS-PAGE and as a 15 kDa band after reduction. The above immunological and biochemical characteristics and subunit structure of coho salmon Vg, E1, and E2 were found to be nearly identical to the corresponding proteins in several other salmonid species of diverse genera. These properties of Vg have been highly conserved during salmonid evolution.
- 社団法人日本動物学会の論文
- 1993-04-15
著者
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Hara A
Laboratory Of Biochemistry Gifu Pharmaceutical University
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Hara A
Gifu Pharmaceutical Univ. Gifu Jpn
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Hara Akihiko
Nanae Fish Culture Experimantal Station Faculty Of Fisheries Hokkaido University
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Sullivan V.
Department Of Zoology North Carolina State University
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Sullivan Craig
Department Of Zoology North Caroline State University
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Hara A
Division Of Marine Biosciences Graduate School Of Fisheries Sciences Hokkaido University
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Dickhoff W.
School Of Fisheries University Of Washington:national Marine Fisheries Service
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Hara Akihiko
Nanae Experimental Aquaculture Station Faculty Of Fisheries Hokkaido University
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Dickhoff Walton
School of Fisheries, University of Washington
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