Secretory Function of the Test Cell in Larval Tunic Formation in the Ascidian Ciona intestinalis:An Immunoelectron Microscopic Study
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概要
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The test cells, one of the two accessory cell groups of the ascidian oocyte, persist around the developing embryos and the hatched tadpole larvae, but their function during embryogenesis is not fully understood. We have produced monoclonal antibodies that specifically recognize the test cells in Ciona intestinalis (Takamura et al., Zool. Sci. 13: 241-251, 1996).In the present paper, we demonstrate the ultrastructural localization of the antigen reactive to one of the test cell-specific antibody UA165 by immunogold labeling. The mature test cells in Ciona intestinalis contain two types of cytoplasmic inclusions, i.e., irregular vacuoles with electron-dense cores and spherical vesicles with a moderately electron-dense content. UA165-immunogold particles specifically labels the content of the spherical vesicles. Concomitantly with larval tunic formation at the early tailbud stage, the spherical vesicles begin to rupture and the UA165 antigen is exocytosed into the perivitelline space surrounding the embryo. The released antigen gradually deposits on the outermost layer (outer cuticular layer) of the larval tunic. Shortly after the beginning of exocytosis, the test cells scatter in the perivitelline space, move to the embryonic surface, and adhere to the outer surface of the larval tunic. These findings indicate that during the tailbud stage the test cells secrete a component that is necessary for the completion of the larval tunic.
- 社団法人日本動物学会の論文
- 1996-04-15
著者
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Okada Toshiaki
Ushimado Marine Laboratory Okayama University
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YAMAMOTO Masamichi
Ushimado Marine Laboratory, Okayama University
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Takamura Katsumi
Department of Marine Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University
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Yamaguchi Yasunori
Department of Biotechnology, Faculty of Engineering, Fukuyama University
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Yamaguchi Yasunori
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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Takamura Katsumi
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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Yamamoto Masamichi
Ushimado Marine Laboratory Faculty Of Science Okayama University
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