Cell Culture of the Antennal Imaginal Disc of the Silkworm, Bombyx mori L. and Differentiation of Neurons from the Culture : Cell Biology

概要

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Antennal imaginal disc of the silkworm larvae is an epidermal organ composed of undifferentiated cells, while during metamorphosis a rapid and remarkable neurogenesis of adult olfactory neurons takes place from the disc. The discs of the 5th instar larvae were excised and disrupted enzymatically or mechanically and then cultured in vitro using some insect culture media. Cells proliferated in these media in various ways, i. e. formation of flat cell sheet, network of cytoplasmic elongations, hollow monolayered vesicles and cell dispersions. Presently attempts to establish the cell lines from the third one, vesicles, are being in progress but still wait for the success. In the last one, cell dispersions, several cell types differentiated from the proliferated cells. Among them, two types refered to 'small and medium-sized round cells' seemed to be neuroblasts. Cytoplasmic processes elongated from those cells forming synapse, varicosities and filopodia which are characteristic to neurons. Such morphological characteristics and the origin of those cells, antennal imaginal disc, strongly suggested that they were differentiated neurons in vitro. The importance of this in vitro neurogenesis was discussed.
社団法人日本動物学会の論文
1990-08-15