キノコ中のエルゴステロールおよびビタミンD_2の定量

概要

論文の詳細を見る
A simplified method for the determination of ergosterol and vitamin D_2 in fungi was established. That is, a fraction containing free sterols and vitamin D_2, which was separated by a preliminary fractionation with a Florisil column, was subjected to high-performance liquid chromatography (HPLC) using a Zorbax ODS colunmn(reversed-phase type) with 95% methanol as a mobile Phase. The two peaks corresponding to vitamin D_2 and ergosterol were clearly observed with separation from other concomitants on the chromatogram of HPLC. The overall recoveries of ergosterol and vitamin D_2 by the proposed method were 95.1±0.7(mean±S.E.) and 94.9±0.4%(mean±S.E), respectively. Then the proposed method was applied to the analysis of ergosterol and vitamin D_2 in fungi, commercial dried Shiitake and Hiratake. The results obtained were satisfactory. In both cases, the sterols were for the most part to exist in free form, and the all of the sterol component was demonstrated to be ergosterol. Vitamin D_2 was present in small amount in both commercial Shiitake and Hiratake and it increased by sun or ultraviolet light irradiation.
社団法人日本家政学会の論文
1990-05-05